Phospho-NFKB2 (Ser866) Antibody (PACO24519)
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주문- SKU:
- PACO24519
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
Antibody Name: | Phospho-NFKB2 (Ser866) Antibody (PACO24519) |
Antibody SKU: | PACO24519 |
Size: | 100ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 |
Species Reactivity: | Human, Mouse, Rat |
Immunogen: | Peptide sequence around phosphorylation site of serine 866(E-D-S(p)-A-Y) derived from Human NF B-p100. |
Form: | Liquid |
Storage Buffer: | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western blot analysis of extracts from ovary cancer cells using NF-κB p100 (phospho-Ser866) antibody. | |
Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using NF-κB p100 (phospho- ser866) antibody. | |
Immunofluorescence staining of methanol-fixed HeLa cells using NF-kappa;B p100 (phospho-ser866) antibody. |
Background: | NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. In a non-canonical activation pathway, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. The NF-kappa-B heterodimeric RelB-p52 complex is a transcriptional activator. The NF-kappa-B p52-p52 homodimer is a transcriptional repressor. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. p52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. p52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. Dobrzanski P., Ryseck R.P., Bravo R.EMBO J. 13:4608-4616(1994) Beg A.A., Baldwin A.S. Jr.Oncogene 9:1487-1492(1994) |
Synonyms: | p52; p105; H2TF1; LYT10; NF-kB2 |
UniProt Protein Function: | NFkB-p100: a transcription factor of the nuclear factor-kappaB ( NFkB) group. Precursor of the p52 subunit of the nuclear factor NF-kappa-B, which binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. The precursor protein itself does not bind to DNA. LT-beta receptor agonists and LPS induce NF-kappaB/p100 processing to p52 at the level of the ribosome. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Two spliced isoforms have been identified. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. |
UniProt Protein Details: | Protein type:Transcription factor; Oncoprotein; DNA-binding Chromosomal Location of Human Ortholog: 10q24 Cellular Component: Bcl3/NF-kappaB2 complex; nucleoplasm; cytoplasm; nucleus; cytosol Molecular Function:protein binding; transcription coactivator activity; chromatin binding; transcription factor activity Biological Process: spleen development; I-kappaB kinase/NF-kappaB cascade; extracellular matrix organization and biogenesis; transcription, DNA-dependent; follicular dendritic cell differentiation; MyD88-independent toll-like receptor signaling pathway; rhythmic process; toll-like receptor 3 signaling pathway; negative regulation of transcription from RNA polymerase II promoter; toll-like receptor 10 signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; regulation of transcription, DNA-dependent; response to cytokine stimulus; toll-like receptor signaling pathway; germinal center formation; positive regulation of interferon type I production; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; inflammatory response; toll-like receptor 4 signaling pathway Disease: Immunodeficiency, Common Variable, 10 |
NCBI Summary: | This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFkB). The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner. The p100 full-length protein is co-translationally processed into a p52 active form. Chromosomal rearrangements and translocations of this locus have been observed in B cell lymphomas, some of which may result in the formation of fusion proteins. There is a pseudogene for this gene on chromosome 18. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013] |
UniProt Code: | Q00653 |
NCBI GenInfo Identifier: | 116242678 |
NCBI Gene ID: | 4791 |
NCBI Accession: | Q00653.4 |
UniProt Secondary Accession: | Q00653,Q04860, Q9BU75, Q9H471, Q9H472, A8K9D9, D3DR83 |
UniProt Related Accession: | Q00653,AAB23437 |
Molecular Weight: | 900 |
NCBI Full Name: | Nuclear factor NF-kappa-B p100 subunit |
NCBI Synonym Full Names: | nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100) |
NCBI Official Symbol: | NFKB2 |
NCBI Official Synonym Symbols: | p52; p100; H2TF1; LYT10; CVID10; LYT-10; NF-kB2 |
NCBI Protein Information: | nuclear factor NF-kappa-B p100 subunit; oncogene Lyt-10; NFKB, p52/p100 subunit; DNA-binding factor KBF2; transcription factor NFKB2; nuclear factor Kappa-B, subunit 2; nuclear factor NF-kappa-B p52 subunit; lymphocyte translocation chromosome 10 protein; nuclear factor of Kappa light chain gene enhancer in B cells 2 |
UniProt Protein Name: | Nuclear factor NF-kappa-B p100 subunit |
UniProt Synonym Protein Names: | DNA-binding factor KBF2; H2TF1; Lymphocyte translocation chromosome 10 protein; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2; Oncogene Lyt-10; Lyt10 |
Protein Family: | Nuclear factor |
UniProt Gene Name: | NFKB2 |
UniProt Entry Name: | NFKB2_HUMAN |
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