Phospho-MYL9 (Ser15) Antibody (PACO24214)
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주문- SKU:
- PACO24214
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | Phospho-MYL9 (Ser15) Antibody (PACO24214) |
Antibody SKU: | PACO24214 |
Size: | 100ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:1000, IF:1:100-1:200 |
Species Reactivity: | Human |
Immunogen: | Peptide sequence around phosphorylation site of serine 15 (A-N-S(p)-N-V) derived from Human MLC2. |
Form: | Liquid |
Storage Buffer: | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Immunofluorescence staining of methanol-fixed U87 cells using MLC2 (Phospho-Ser15) Antibody. | |
Background: | Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion. 1) Xia, Y. et al. c-Jun downregulation by HDAC3-dependent transcriptional repression promotes osmotic stress-induced cell apoptosis. Mol. Cell 25, 219–232 (2007). 2)Vander Heiden, M. G. et al. Evidence for an alternative glycolytic pathway in rapidly proliferating cells. Science 329, 1492–1499 (2010). 3) Fang, D. et al. Phosphorylation of beta-catenin by AKT promotes beta-catenin transcriptional activity. J. Biol. Chem. 282, 11221–11229 (2007). |
Synonyms: | MLC2; MRLC1; MYRL2 |
UniProt Protein Function: | MRLC1: Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion. Myosin is an hexamer of 2 heavy chains and 4 light chains. Smooth muscle tissues and in some, but not all, nonmuscle cells. 2 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Contractile; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 20q11.23 Cellular Component: cytosol; muscle myosin complex; stress fiber; Z disc Molecular Function:calcium ion binding; myosin heavy chain binding; structural constituent of muscle Biological Process: axon guidance; ephrin receptor signaling pathway; muscle contraction; regulation of muscle contraction; small GTPase mediated signal transduction |
NCBI Summary: | Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
UniProt Code: | P24844 |
NCBI GenInfo Identifier: | 20141521 |
NCBI Gene ID: | 10398 |
NCBI Accession: | P24844.4 |
UniProt Secondary Accession: | P24844,Q9BQL9, Q9BUF9, Q9H136, E1P5T6, |
UniProt Related Accession: | P24844 |
Molecular Weight: | 13,866 Da |
NCBI Full Name: | Myosin regulatory light polypeptide 9 |
NCBI Synonym Full Names: | myosin light chain 9 |
NCBI Official Symbol: | MYL9 |
NCBI Official Synonym Symbols: | LC20; MLC2; MRLC1; MYRL2; MLC-2C |
NCBI Protein Information: | myosin regulatory light polypeptide 9 |
UniProt Protein Name: | Myosin regulatory light polypeptide 9 |
UniProt Synonym Protein Names: | 20 kDa myosin light chain; LC20; MLC-2C; Myosin RLC; Myosin regulatory light chain 2, smooth muscle isoform; Myosin regulatory light chain 9; Myosin regulatory light chain MRLC1 |
Protein Family: | Myosin regulatory light polypeptide |
UniProt Gene Name: | MYL9 |
UniProt Entry Name: | MYL9_HUMAN |
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