Phospho-EIF4EBP1 (Thr36) Antibody (PACO24361)
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주문- SKU:
- PACO24361
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
Antibody Name: | Phospho-EIF4EBP1 (Thr36) Antibody (PACO24361) |
Antibody SKU: | PACO24361 |
Size: | 100ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:1000, IHC:1:50-1:100 |
Species Reactivity: | Human, Mouse, Rat |
Immunogen: | Peptide sequence around phosphorylation site of threonine 36(S-T-T(p)-P-G) derived from Human 4E-BP1. |
Form: | Liquid |
Storage Buffer: | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western blot analysis of extracts from MDA-MB-435 cells, untreated or EGF-treated (200 ng/ml, 30min), using 4E-BP1 (Ab-36) antibody (Lane 1 and 2) and 4E-BP1 (phospho-Thr36) antibody (Lane 3 and 4). | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using 4E-BP1 (phospho-Thr36) antibody. |
Background: | Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways. |
Synonyms: | BP-1; 4EBP1; 4E-BP1; PHAS-I; |
UniProt Protein Function: | 4E-BP1: binds to eIF4E, preventing its assembly into the EIF4F complex and inhibiting cap-dependent translation. Phosphorylation of 4E-BP1 disrupts this binding, activating cap-dependent translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the PI3 kinase pathway. |
UniProt Protein Details: | Protein type:Translation; Translation initiation Chromosomal Location of Human Ortholog: 8p12 Cellular Component: nucleoplasm; protein complex; cytoplasm; cytosol Molecular Function:protein binding; translation repressor activity; eukaryotic initiation factor 4E binding Biological Process: negative regulation of translational initiation; TOR signaling pathway; response to ethanol; cellular protein metabolic process; translation; translational initiation; insulin receptor signaling pathway; gene expression; positive regulation of mitotic cell cycle; negative regulation of protein complex assembly; G1/S transition of mitotic cell cycle; lung development |
NCBI Summary: | This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008] |
UniProt Code: | Q13541 |
NCBI GenInfo Identifier: | 34921508 |
NCBI Gene ID: | 1978 |
NCBI Accession: | Q13541.3 |
UniProt Secondary Accession: | Q13541,Q6IBN3, B2R502, D3DSW8, |
UniProt Related Accession: | Q13541 |
Molecular Weight: | 118 |
NCBI Full Name: | Eukaryotic translation initiation factor 4E-binding protein 1 |
NCBI Synonym Full Names: | eukaryotic translation initiation factor 4E binding protein 1 |
NCBI Official Symbol: | EIF4EBP1 |
NCBI Official Synonym Symbols: | BP-1; 4EBP1; 4E-BP1; PHAS-I |
NCBI Protein Information: | eukaryotic translation initiation factor 4E-binding protein 1; eIF4E-binding protein 1; phosphorylated heat- and acid-stable protein regulated by insulin 1 |
UniProt Protein Name: | Eukaryotic translation initiation factor 4E-binding protein 1 |
UniProt Synonym Protein Names: | Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I |
Protein Family: | Eukaryotic translation initiation factor 4E-binding protein |
UniProt Gene Name: | EIF4EBP1 |
UniProt Entry Name: | 4EBP1_HUMAN |
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