Phospho-DOK1 (Tyr398) Antibody (PACO24320)
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주문- SKU:
- PACO24320
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | Phospho-DOK1 (Tyr398) Antibody (PACO24320) |
Antibody SKU: | PACO24320 |
Size: | 100ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:1000, IHC:1:50-1:100 |
Species Reactivity: | Human, Mouse, Rat |
Immunogen: | Peptide sequence around phosphorylation site of tyrosine 398 (E-G-Y(p)-E-L) derived from Human p62Dok. |
Form: | Liquid |
Storage Buffer: | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western blot analysis of extracts from K562 cells untreated or treated with H2O2 using p62Dok(phospho-Tyr398) Antibody. | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p62Dok(Phospho-Tyr398) Antibody(left) or the same antibody preincubated with blocking peptide(right). |
Background: | DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3. |
Synonyms: | DOK1 |
UniProt Protein Function: | DOK1: a docking protein that interacts with receptor tyrosine kinases. It is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. Docking protein 1 contains a putative pleckstrin homology domain at the amino terminus and ten PXXP SH3 recognition motifs. Docking protein 2 binds p120 (RasGAP) from CML cells. It has been postulated to play a role in mitogenic signaling. |
UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Adaptor/scaffold Chromosomal Location of Human Ortholog: 2p13 Cellular Component: perinuclear region of cytoplasm; cytoplasm; cytosol; nucleus Molecular Function:protein binding; receptor signaling protein activity; insulin receptor binding Biological Process: cell surface receptor linked signal transduction; Ras protein signal transduction; insulin receptor signaling pathway; signal transduction; transmembrane receptor protein tyrosine kinase signaling pathway |
NCBI Summary: | The protein encoded by this gene is part of a signal transduction pathway downstream of receptor tyrosine kinases. The encoded protein is a scaffold protein that helps form a platform for the assembly of multiprotein signaling complexes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2010] |
UniProt Code: | Q99704 |
NCBI GenInfo Identifier: | 17366642 |
NCBI Gene ID: | 1796 |
NCBI Accession: | Q99704.1 |
UniProt Secondary Accession: | Q99704,O43204, Q53TY2, Q9UHG6, |
UniProt Related Accession: | Q99704 |
Molecular Weight: | 481 |
NCBI Full Name: | Docking protein 1 |
NCBI Synonym Full Names: | docking protein 1, 62kDa (downstream of tyrosine kinase 1) |
NCBI Official Symbol: | DOK1 |
NCBI Official Synonym Symbols: | P62DOK |
NCBI Protein Information: | docking protein 1; pp62; p62(dok); Downstream of tyrosine kinase 1; docking protein 1 (downstream of tyrosine kinase 1) |
UniProt Protein Name: | Docking protein 1 |
UniProt Synonym Protein Names: | Downstream of tyrosine kinase 1; p62(dok); pp62 |
Protein Family: | Docking protein |
UniProt Gene Name: | DOK1 |
UniProt Entry Name: | DOK1_HUMAN |
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