Phospho-CEBPA (Ser21) Antibody (PACO24366)
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주문- SKU:
- PACO24366
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
Antibody Name: | Phospho-CEBPA (Ser21) Antibody (PACO24366) |
Antibody SKU: | PACO24366 |
Size: | 100ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:1000, IHC:1:50-1:100 |
Species Reactivity: | Human, Mouse, Rat |
Immunogen: | Peptide sequence around phosphorylation site of Serine 21 (L-Q-S(p)-P-P) derived from Human C/EBP- α . |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western blot analysis of extracts from HepG2 cells treated with EGF using C/EBP-alpha (phospho-Ser21) Antibody.The lane on the right is treated with the antigen-specific peptide. | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using C/EBP- alpha (phospho-Ser21) antibody (left)or the same antibody preincubated with blocking peptide (right). |
Background: | C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. |
Synonyms: | CAP4; ICE8; MACH; MCH5; Mch-5 |
UniProt Protein Function: | C/EBP-alpha: a bZIP transcription factor which can form homodimers or heterodimers with the related proteins CEBP-beta and CEBP-gamma. Binds to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells. |
UniProt Protein Details: | Protein type:DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 19q13.1 Cellular Component: Rb-E2F complex; nuclear matrix; nucleus Molecular Function:RNA polymerase II transcription factor activity, enhancer binding; protein binding; protein homodimerization activity; DNA binding; protein heterodimerization activity; protein complex binding; transcription factor activity; transcription factor binding Biological Process: transcription from RNA polymerase II promoter; fat cell differentiation; embryonic placenta development; macrophage differentiation; viral reproduction; cell maturation; response to glucocorticoid stimulus; negative regulation of transcription from RNA polymerase II promoter; glucose homeostasis; negative regulation of cell proliferation; response to vitamin B2; acute-phase response; inner ear development; cholesterol metabolic process; mitochondrion organization and biogenesis; generation of precursor metabolites and energy; organ regeneration; granulocyte differentiation; transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; negative regulation of cyclin-dependent protein kinase activity; liver development; positive regulation of osteoblast differentiation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; white fat cell differentiation; brown fat cell differentiation; positive regulation of fat cell differentiation; positive regulation of transcription from RNA polymerase III promoter; myeloid cell differentiation; positive regulation of transcription from RNA polymerase II promoter; urea cycle; lung development Disease: Leukemia, Acute Myeloid |
NCBI Summary: | This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. Mutation of this gene is associated with acute myeloid leukemia. The use of alternative in-frame non-AUG (GUG) and AUG start codons results in protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the GUG and the first AUG start codons. [provided by RefSeq, Dec 2013] |
UniProt Code: | P49715 |
NCBI GenInfo Identifier: | 166898082 |
NCBI Gene ID: | 1050 |
NCBI Accession: | P49715.3 |
UniProt Secondary Accession: | P49715,P78319, Q05CA4, A7LNP2, |
UniProt Related Accession: | P49715 |
Molecular Weight: | 37,561 Da |
NCBI Full Name: | CCAAT/enhancer-binding protein alpha |
NCBI Synonym Full Names: | CCAAT/enhancer binding protein (C/EBP), alpha |
NCBI Official Symbol: | CEBPA |
NCBI Official Synonym Symbols: | CEBP; C/EBP-alpha |
NCBI Protein Information: | CCAAT/enhancer-binding protein alpha |
UniProt Protein Name: | CCAAT/enhancer-binding protein alpha |
Protein Family: | CCAAT/enhancer-binding protein |
UniProt Gene Name: | CEBPA |
UniProt Entry Name: | CEBPA_HUMAN |
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