Phospho-CDC25A (Ser178) Antibody (PACO24036)
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주문- SKU:
- PACO24036
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
Antibody Name: | Phospho-CDC25A (Ser178) Antibody (PACO24036) |
Antibody SKU: | PACO24036 |
Size: | 100ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:1000 |
Species Reactivity: | Human, Mouse, Rat |
Immunogen: | Peptide sequence around phosphorylation site of serine 178(Q-N-S(p)-A-P) derived from Human CDC25A . |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western blot analysis of extracts from A549 cells treated with UV using Phospho-CDC25A (Ser178) antibody.The lane on the right is treated with the antigen-specific peptide. |
Background: | Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDC2 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro. |
Synonyms: | CDC25M3; MPIP1; Dual specificity phosphatase Cdc25A; M-phase inducer phosphatase 1; |
UniProt Protein Function: | CDC25A: a member of the MPI phosphatase family. Functions as a dosage-dependent inducer in mitotic control. Required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two splice variant isoforms have been described. |
UniProt Protein Details: | Protein type:Protein phosphatase, dual-specificity; EC 3.1.3.48; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 3p21 Cellular Component: nucleoplasm; cytosol Molecular Function:protein binding; protein tyrosine phosphatase activity; protein kinase binding Biological Process: cell proliferation; mitosis; response to radiation; cell division; regulation of cell cycle; mitotic cell cycle; regulation of cyclin-dependent protein kinase activity; DNA replication; G2/M transition of mitotic cell cycle; G1/S transition of mitotic cell cycle |
NCBI Summary: | CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
UniProt Code: | P30304 |
NCBI GenInfo Identifier: | 50403734 |
NCBI Gene ID: | 993 |
NCBI Accession: | P30304.2 |
UniProt Secondary Accession: | P30304,Q8IZH5, Q96IL3, Q9H2F2, |
UniProt Related Accession: | P30304 |
Molecular Weight: | |
NCBI Full Name: | M-phase inducer phosphatase 1 |
NCBI Synonym Full Names: | cell division cycle 25A |
NCBI Official Symbol: | CDC25A |
NCBI Official Synonym Symbols: | CDC25A2 |
NCBI Protein Information: | M-phase inducer phosphatase 1; CDC25A2-CAG isoform; CDC25 isoform A1-CAG; dual specificity phosphatase CDC25A |
UniProt Protein Name: | M-phase inducer phosphatase 1 |
UniProt Synonym Protein Names: | Dual specificity phosphatase Cdc25A |
Protein Family: | M-phase inducer phosphatase |
UniProt Gene Name: | CDC25A |
UniProt Entry Name: | MPIP1_HUMAN |
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