PFKM Antibody (PACO47590)
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주문- SKU:
- PACO47590
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
Antibody Name: | PFKM Antibody (PACO47590) |
Antibody SKU: | PACO47590 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
Species Reactivity: | Human, Mouse |
Immunogen: | Recombinant Human ATP-dependent 6-phosphofructokinase, muscle type protein (166-334AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: A549 whole cell lysate, Mouse heart tissue. All lanes: PFKM antibody at 3.4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 86, 82, 94 kDa. Observed band size: 86 kDa.. | |
Immunofluorescent analysis of A549 cells using PACO47590 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). | |
Immunohistochemistry of paraffin-embedded human endometrial cancer using PACO47590 at dilution of 1:100. |
Background: | Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis. |
Synonyms: | ATP-dependent 6-phosphofructokinase, muscle type (ATP-PFK) (PFK-M) (EC 2.7.1.11) (6-phosphofructokinase type A) (Phosphofructo-1-kinase isozyme A) (PFK-A) (Phosphohexokinase), PFKM, PFKX |
UniProt Protein Function: | PFKM: phosphofructokinase, muscle type. An ubiquitous metabolic enzyme involved in the synthesis and degradation of fructose 2,6-bisphosphate. Key control step of glycolysis. An allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate. Activity: ATP D-fructose 6-phosphate = ADP D-fructose 1,6-bisphosphate. The holoenzyme consists of 4 subunits. The liver and muscle enzymes are homo-tetramers of four liver or muscle isoforms, respectively. The red blood cell enzyme consists hetero-tetramers of the muscle and liver isoforms. A subunit composition with a higher proportion of platelet type subunits is found in platelets, brain and fibroblasts. Defects in PFKM are the cause of glycogen storage disease VII (GSD-VII) also known as Tarui disease. Two alternatively spliced isoforms have been described. |
UniProt Protein Details: | Protein type:Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - pentose phosphate pathway; EC 2.7.1.11; Carbohydrate Metabolism - fructose and mannose; Kinase, other Chromosomal Location of Human Ortholog: 12q13.3 Cellular Component: 6-phosphofructokinase complex; apical plasma membrane; cytosol Molecular Function:6-phosphofructokinase activity; ATP binding; identical protein binding; kinase binding; metal ion binding; protein binding; protein C-terminus binding; protein homodimerization activity Biological Process: carbohydrate metabolic process; carbohydrate phosphorylation; fructose 6-phosphate metabolic process; glucose homeostasis; glucose metabolic process; glycogen catabolic process; glycolysis; muscle maintenance; positive regulation of insulin secretion; protein oligomerization Disease: Glycogen Storage Disease Vii |
NCBI Summary: | Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Tetramer composition varies depending on tissue type. This gene encodes the muscle-type isozyme. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Alternatively spliced transcript variants have been described.[provided by RefSeq, Nov 2009] |
UniProt Code: | P08237 |
NCBI GenInfo Identifier: | 125126 |
NCBI Gene ID: | 5213 |
NCBI Accession: | P08237.2 |
UniProt Secondary Accession: | P08237,Q16814, Q16815, Q6ZTT1, J3KNX3, |
UniProt Related Accession: | P08237 |
Molecular Weight: | 93,254 Da |
NCBI Full Name: | ATP-dependent 6-phosphofructokinase, muscle type |
NCBI Synonym Full Names: | phosphofructokinase, muscle |
NCBI Official Symbol: | PFKM |
NCBI Official Synonym Symbols: | GSD7; PFK1; PFKA; PFKX; PFK-1; ATP-PFK; PPP1R122 |
NCBI Protein Information: | ATP-dependent 6-phosphofructokinase, muscle type |
UniProt Protein Name: | ATP-dependent 6-phosphofructokinase, muscle type |
UniProt Synonym Protein Names: | 6-phosphofructokinase type A; Phosphofructo-1-kinase isozyme A; PFK-A; Phosphohexokinase |
Protein Family: | ATP-dependent 6-phosphofructokinase |
UniProt Gene Name: | PFKM |
UniProt Entry Name: | PFKAM_HUMAN |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
Recommended Products |
Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |