PCCB Antibody (PACO57912)
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주문- SKU:
- PACO57912
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | PCCB Antibody (PACO57912) |
Antibody SKU: | PACO57912 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Propionyl-CoA carboxylase β chain, mitochondrial protein (200-314AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: Jurkat whole cell lysate. All lanes: PCCB antibody at 7.7µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 59, 61 kDa. Observed band size: 59 kDa. | |
IHC image of PACO57912 diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
IHC image of PACO57912 diluted at 1:300 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
Background: | cytosol, mitochondrial matrix, mitochondrion, CoA carboxylase activity, propionyl-CoA carboxylase activity, biotin metabolic process, fatty acid, catabolic process, short-chain fatty acid, catabolic process |
Synonyms: | Propionyl-CoA carboxylase β chain, mitochondrial (PCCase subunit beta) (EC 6.4.1.3) (Propanoyl-CoA:carbon dioxide ligase subunit beta), PCCB |
UniProt Protein Function: | PCCB: Defects in PCCB are the cause of propionic acidemia type II (PA-2). PA-2 is a life-threatening disease characterized by episodic vomiting, lethargy and ketosis, neutropenia, periodic thrombocytopenia, hypogammaglobulinemia, developmental retardation, and intolerance to protein. Belongs to the AccD/PCCB family. 2 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:EC 6.4.1.3; Ligase; Carbohydrate Metabolism - propanoate; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Mitochondrial Chromosomal Location of Human Ortholog: 3q21-q22 Cellular Component: mitochondrion; mitochondrial matrix; cytosol Molecular Function:propionyl-CoA carboxylase activity; ATP binding Biological Process: vitamin metabolic process; fatty acid beta-oxidation; short-chain fatty acid catabolic process; cellular lipid metabolic process; biotin metabolic process; water-soluble vitamin metabolic process Disease: Propionic Acidemia |
NCBI Summary: | The protein encoded by this gene is a subunit of the propionyl-CoA carboxylase (PCC) enzyme, which is involved in the catabolism of propionyl-CoA. PCC is a mitochondrial enzyme that probably acts as a dodecamer of six alpha subunits and six beta subunits. This gene encodes the beta subunit of PCC. Defects in this gene are a cause of propionic acidemia type II (PA-2). Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010] |
UniProt Code: | P05166 |
NCBI GenInfo Identifier: | 124106304 |
NCBI Gene ID: | 5096 |
NCBI Accession: | P05166.3 |
UniProt Secondary Accession: | P05166,Q16813, Q96CX0, B7Z2Z4, |
UniProt Related Accession: | P05166 |
Molecular Weight: | 60,521 Da |
NCBI Full Name: | Propionyl-CoA carboxylase beta chain, mitochondrial |
NCBI Synonym Full Names: | propionyl CoA carboxylase, beta polypeptide |
NCBI Official Symbol: | PCCB |
NCBI Protein Information: | propionyl-CoA carboxylase beta chain, mitochondrial; PCCase subunit beta; propanoyl-CoA:carbon dioxide ligase subunit beta; propionyl Coenzyme A carboxylase, beta polypeptide |
UniProt Protein Name: | Propionyl-CoA carboxylase beta chain, mitochondrial |
UniProt Synonym Protein Names: | Propanoyl-CoA:carbon dioxide ligase subunit beta |
Protein Family: | Phycoerythrocyanin |
UniProt Gene Name: | PCCB |
UniProt Entry Name: | PCCB_HUMAN |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
Recommended Products |
Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |