NSMAF Antibody (PACO62795)
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주문- SKU:
- PACO62795
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | NSMAF Antibody (PACO62795) |
Antibody SKU: | PACO62795 |
Size: | 50ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Protein FAN protein (1-233AA ) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
IHC image of PACO62795 diluted at 1:300 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
Immunofluorescence staining of A549 cells with PACO62795 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
Background: | Couples the p55 TNF-receptor (TNF-R55 / TNFR1) to neutral sphingomyelinase (N-SMASE). Specifically binds to the N-smase activation domain of TNF-R55. May regulate ceramide production by N-SMASE. |
Synonyms: | Protein FAN (Factor associated with neutral sphingomyelinase activation) (Factor associated with N-SMase activation), NSMAF, FAN |
UniProt Protein Function: | Couples the p55 TNF-receptor (TNF-R55 / TNFR1) to neutral sphingomyelinase (N-SMASE). Specifically binds to the N-smase activation domain of TNF-R55. May regulate ceramide production by N-SMASE. |
NCBI Summary: | This gene encodes a WD-repeat protein that binds the cytoplasmic sphingomyelinase activation domain of the 55kD tumor necrosis factor receptor. This protein is required for TNF-mediated activation of neutral sphingomyelinase and may play a role in regulating TNF-induced cellular responses such as inflammation. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2009] |
UniProt Code: | Q92636 |
NCBI GenInfo Identifier: | 209572614 |
NCBI Gene ID: | 8439 |
NCBI Accession: | Q92636.2 |
UniProt Secondary Accession: | Q92636,Q8IW26, B4DFB0, E9PCH0, |
UniProt Related Accession: | Q92636 |
Molecular Weight: | 107,314 Da |
NCBI Full Name: | Protein FAN |
NCBI Synonym Full Names: | neutral sphingomyelinase activation associated factor |
NCBI Official Symbol: | NSMAF |
NCBI Official Synonym Symbols: | FAN; GRAMD5 |
NCBI Protein Information: | protein FAN |
UniProt Protein Name: | Protein FAN |
UniProt Synonym Protein Names: | Factor associated with neutral sphingomyelinase activation; Factor associated with N-SMase activation |
Protein Family: | Protein |
UniProt Gene Name: | NSMAF |
UniProt Entry Name: | FAN_HUMAN |
Antibodies |
Anti-NSMAF Antibody (CAB15742) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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Anti-HRP-conjugated Beta Actin Antibody (CABC028) |