NELFB Antibody (PACO31744)
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주문- SKU:
- PACO31744
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- ChIP
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | NELFB Antibody (PACO31744) |
Antibody SKU: | PACO31744 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC, ChIP |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:20-1:200 |
Species Reactivity: | Human, Mouse |
Immunogen: | Recombinant Human Negative elongation factor B protein (8-199AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western Blot All lanes: NELFB antibody at 2µg/ml Lane 1: NIH/3T3 whole cell lysate Lane 2: HL60 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 66 kDa Observed band size: 66 kDa . | |
Immunohistochemistry of paraffin-embedded human colon cancer using PACO31744 at dilution of 1:100. | |
Immunohistochemistry of paraffin-embedded human endometrial cancer using PACO31744 at dilution of 1:100. |
Background: | Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. Binds RNA which may help to stabilize the NELF complex on nucleic acid, In vitro, binds weakly to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1. May be able to induce chromatin unfolding. |
Synonyms: | Negative elongation factor B (NELF-B) (Cofactor of BRCA1), NELFB, COBRA1 KIAA1182 |
UniProt Protein Function: | COBRA1: a subunit of the multisubunit transcription elongation factor NELF (negative elongation factor). May be involved in transcriptional pausing of RNA polymerase II (RNAPII). Interacts with the first BRCT repeat of BRCA1. |
UniProt Protein Details: | Protein type:Nuclear receptor co-regulator; Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 9q34 Cellular Component: nucleoplasm; cytoplasm Molecular Function:protein binding Biological Process: transcription from RNA polymerase II promoter; viral reproduction; positive regulation of viral transcription; RNA elongation from RNA polymerase II promoter; gene expression; negative regulation of transcription, DNA-dependent |
NCBI Summary: | NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2; MIM 606026), either NELFC or NELFD (TH1L; MIM 605297), and NELFE (RDBP; MIM 154040). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to cause transcriptional pausing of RNA polymerase II (see MIM 180660) (Narita et al., 2003 [PubMed 12612062]).[supplied by OMIM, Mar 2008] |
UniProt Code: | Q8WX92 |
NCBI GenInfo Identifier: | 38372378 |
NCBI Gene ID: | 25920 |
NCBI Accession: | Q8WX92.1 |
UniProt Related Accession: | Q8WX92 |
Molecular Weight: | 65kDa |
NCBI Full Name: | Negative elongation factor B |
NCBI Synonym Full Names: | negative elongation factor complex member B |
NCBI Official Symbol: | NELFB |
NCBI Official Synonym Symbols: | COBRA1; NELF-B |
NCBI Protein Information: | negative elongation factor B |
UniProt Protein Name: | Negative elongation factor B |
UniProt Synonym Protein Names: | Cofactor of BRCA1 |
UniProt Gene Name: | NELFB |
UniProt Entry Name: | NELFB_HUMAN |
Antibodies |
Anti-NELFB Antibody (CAB12023) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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