TEK, or TIE-2, is an endothelial cell-specific receptor tyrosine kinase (RTK) that is known as a functioning molecule of vascular endothelial cells. TEK comprises a subfamily of RTK with TIE, and these two receptors play critical roles in vascular maturation, maintenance of integrity and remodeling. Targeted mutagenesis of both Tek and its agonistic ligand, Angiopoietin-1, result in embryonic lethality, demonstrating that the signal transduction pathways mediated by this receptor are crucial for normal embryonic development. TEK signaling is indispensable for the development of the embryonic vasculature and suggests that TEK signaling may also be required for the development of the tumor vasculature.
Product Name:
Mouse Tie2/CD202b Recombinant Protein (RPES2884)
Product Code:
RPES2884
Size:
20µg
Species:
Mouse
Expressed Host:
Baculovirus-Insect Cells
Synonyms:
AA517024,Cd202b,Hyk,STK1,Tie-2,Tie2
Accession:
Q02858
Sequence:
Gln770-Ala1122
Fusion tag:
N-His-GST
Activity:
1. No Kinase Activity2. Measured by its binding ability in a functional ELISA. Immobilized mouse TEK (aa 770-1122) at 2 µg/ml (100 ?l/well) can bind human Ang2-Fc with a linear range of 0.25-2.0 µg/ml.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the mouse TEK (Q02858) (Gln770-Ala1122) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
