Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. PD-L1/B7-H1 is a member of the growing B7 family of immune molecules and this protein contains one V-like and one C-like Ig domain within the extracellular domain, and together with PD-L2, are two ligands for PD1 which belongs to the CD28/CTLA4 family expressed on activated lymphoid cells. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.Immune CheckpointImmune Checkpoint Blockade: Blocking Antibodies Immune Checkpoint Blockade: PD-L1 / B7-H1 / CD274 Blocking AntibodiesImmune Checkpoint Detection: Antibodies Immune Checkpoint Detection: ELISA Antibodies Immune Checkpoint Detection: IHC Antibodies Immune Checkpoint Detection: ICC Antibodies Immune Checkpoint Detection: FCM Antibodies Immune Checkpoint Detection: WB AntibodiesImmune Checkpoint Proteins PD-L1 / B7-H1 / CD274 Immune Checkpoint ProteinsImmune Checkpoint Targets Co-inhibitory Immune Checkpoint Targets Immunotherapy Cancer Immunotherapy Targeted Therapy
Product Name:
Mouse PD-L1/B7-H1/CD274 Recombinant Protein (RPES4258)
Product Code:
RPES4258
Size:
100µg
Species:
Mouse
Expressed Host:
HEK293 Cells
Synonyms:
Programmed cell death 1 ligand 1Cd274, programmed cell death 1 ligand 1,PD-L1,PDCD1 ligand 1,programmed death ligand 1,B7 homolog 1,B7-H1,CD274,B7h1,Pdcd1l1,Pdcd1lg1,Pdl1
Accession:
NP_068693.1
Sequence:
Met 1-Thr 238
Fusion tag:
C-His-Fc
Activity:
Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse PD-1 at 10 µg/ml (100 ?l/well) can bind biotinylated mouse PD-L1 with a linear range of 3.2-400 ng/ml.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the extracellular domain (Met 1-Thr 238) of mouse PD-L1 (NP_068693.1) was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus.
Purity:
> 97 % as determined by SDS-PAGE
Mol Mass:
52.8 kDa
AP Mol Mass:
65-75 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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