Mouse interleukin 13 (mIL-13) is a pleiotropic cytokine produced by activated Th2 cells. IL-13 induces B cell proliferation and immunoglobin production. It contains a four helical bundle with two internal disulfide bonds. Mouse IL13 shares 58% sequence identity with human protein and exhibits cross-species activity. IL13 signals via receptor IL13R (type2, IL4R) and activates STAT-6. IL13 initially binds IL-13R?1 with low affinity and triggers association of IL4R?, generating a high affinity heterodimeric receptor IL13R and eliciting downstream signals. IL13 also binds IL-13R?2 with high affinity, which plays a role in a negative feedback system of IL13 signaling. IL13 is an important mediator of allergic inflammation and disease.
Product Name:
Mouse Interleukin3/IL3 Recombinant Protein (RPES1606)
Product Code:
RPES1606
Size:
10µg
Species:
Mouse
Expressed Host:
Human Cells
Synonyms:
Interleukin-13, IL-13, T-Cell Activation Protein P600, Il13, Il-13
Accession:
P20109
Sequence:
Ser26-Phe131
Fusion tag:
C-6His
Activity:
Measured in a cell proliferation assay using TF?1 human erythroleukemic cells. The ED50 for this effect is 2-13 ng/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Mouse Interleukin-13 is produced by our Mammalian expression system and the target gene encoding Ser26-Phe131 is expressed with a 6His tag at the C-terminus.
Purity:
> 95 % as determined by SDS-PAGE
Mol Mass:
12.7 kDa
AP Mol Mass:
15-30 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
