MOCS3 Antibody (PACO44659)
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주문- SKU:
- PACO44659
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | MOCS3 Antibody (PACO44659) |
Antibody SKU: | PACO44659 |
Size: | 50ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:20-1:200 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Adenylyltransferase and sulfurtransferase MOCS3 protein (271-460AA) |
Form: | Liquid |
Storage Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Purification Method: | Antigen Affinity Purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western blot. All lanes: MOCS3 antibody at 4.25µg/ml. Lane 1: A549 whole cell lysate. Lane 2: HepG2 whole cell lysate. Lane 3: Jurkat whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 50 kDa. Observed band size: 50 kDa.. | |
Immunohistochemistry of paraffin-embedded human brain tissue using PACO44659 at dilution of 1:100. | |
Immunohistochemistry of paraffin-embedded human liver tissue using PACO44659 at dilution of 1:100. |
Background: | Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions. |
Synonyms: | Adenylyltransferase and sulfurtransferase MOCS3 (Molybdenum cofactor synthesis protein 3) (Molybdopterin synthase sulfurylase) (MPT synthase sulfurylase) [Includes: Molybdopterin-synthase adenylyltransferase (EC 2.7.7.80) (Adenylyltransferase MOCS3) (Sulfur carrier protein MOCS2A adenylyltransferase); Molybdopterin-synthase sulfurtransferase (EC 2.8.1.11) (Sulfur carrier protein MOCS2A sulfurtransferase) (Sulfurtransferase MOCS3)], MOCS3, UBA4 |
UniProt Protein Function: | MOCS3: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions. |
UniProt Protein Details: | Protein type:EC 2.8.1.11; EC 2.7.7.80; Ligase; Transferase Chromosomal Location of Human Ortholog: 20q13.13 Cellular Component: cytosol Molecular Function:sulfurtransferase activity; protein binding; thiosulfate sulfurtransferase activity; metal ion binding; URM1 activating enzyme activity; ATP binding; nucleotidyltransferase activity Biological Process: tRNA wobble uridine modification; protein urmylation; vitamin metabolic process; enzyme active site formation via L-cysteine persulfide; protein amino acid adenylylation; Mo-molybdopterin cofactor biosynthetic process; molybdopterin cofactor biosynthetic process; water-soluble vitamin metabolic process |
NCBI Summary: | Molybdenum cofactor (MoCo) is necessary for the function of all molybdoenzymes. The protein encoded by this gene adenylates and activates molybdopterin synthase, an enzyme required for biosynthesis of MoCo. This gene contains no introns. A pseudogene of this gene is present on chromosome 14. [provided by RefSeq, Nov 2012] |
UniProt Code: | O95396 |
NCBI GenInfo Identifier: | 22001810 |
NCBI Gene ID: | 27304 |
NCBI Accession: | O95396.1 |
UniProt Related Accession: | O95396 |
Molecular Weight: | 460 |
NCBI Full Name: | Adenylyltransferase and sulfurtransferase MOCS3 |
NCBI Synonym Full Names: | molybdenum cofactor synthesis 3 |
NCBI Official Symbol: | MOCS3 |
NCBI Official Synonym Symbols: | UBA4 |
NCBI Protein Information: | adenylyltransferase and sulfurtransferase MOCS3; MPT synthase sulfurylase; molybdopterin synthase sulfurylase; molybdenum cofactor synthesis protein 3; ubiquitin-like modifier activating enzyme 4; UBA4, ubiquitin-activating enzyme E1 homolog |
UniProt Protein Name: | Adenylyltransferase and sulfurtransferase MOCS3 |
UniProt Synonym Protein Names: | Molybdenum cofactor synthesis protein 3; Molybdopterin synthase sulfurylase; Adenylyltransferase MOCS3; Sulfur carrier protein MOCS2A adenylyltransferase; ; Sulfur carrier protein MOCS2A sulfurtransferase; Sulfurtransferase MOCS3 |
UniProt Gene Name: | MOCS3 |
UniProt Entry Name: | MOCS3_HUMAN |