MKLN1 Antibody (PACO60432)
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주문- SKU:
- PACO60432
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | MKLN1 Antibody (PACO60432) |
Antibody SKU: | PACO60432 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200 |
Species Reactivity: | Human, Rat |
Immunogen: | Recombinant Human Muskelin protein (488-614AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: Hela whole cell lysate, 293T whole cell lysate, K562 whole cell lysate, Rat brain tissue. All lanes: MKLN1 antibody at 2.7µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 85, 61 kDa. Observed band size: 85 kDa. | |
Immunofluorescence staining of HepG2 cells with PACO60432 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). | |
IHC image of PACO60432 diluted at 1:400 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
Background: | Acts as a mediator of cell spreading and cytoskeletal responses to the extracellular matrix component THBS1. |
Synonyms: | Muskelin, MKLN1 |
UniProt Protein Function: | MKLN1: Acts as a mediator of cell spreading and cytoskeletal responses to the extracellular matrix component THBS1. 2 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Cell adhesion Chromosomal Location of Human Ortholog: 7q32 Cellular Component: cell cortex; cytosol; ruffle Biological Process: actin cytoskeleton reorganization; cell-matrix adhesion; regulation of cell shape |
NCBI Summary: | Muskelin is an intracellular protein that acts as a mediator of cell spreading and cytoskeletal responses to the extracellular matrix component thrombospondin I (MIM 188060) (Adams et al., 1998 [PubMed 9724633]).[supplied by OMIM, Mar 2008] |
UniProt Code: | Q9UL63 |
NCBI GenInfo Identifier: | 23821862 |
NCBI Gene ID: | 4289 |
NCBI Accession: | Q9UL63.2 |
UniProt Secondary Accession: | Q9UL63,Q9NSK4, Q9NUS8, A4D1M8, A6NG43, |
UniProt Related Accession: | Q9UL63 |
Molecular Weight: | 60,739 Da |
NCBI Full Name: | Muskelin |
NCBI Synonym Full Names: | muskelin 1 |
NCBI Official Symbol: | MKLN1 |
NCBI Official Synonym Symbols: | TWA2 |
NCBI Protein Information: | muskelin |
UniProt Protein Name: | Muskelin |
Protein Family: | Muskelin |
UniProt Gene Name: | MKLN1 |
UniProt Entry Name: | MKLN1_HUMAN |