MCM4 Antibody (PACO18210)
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주문- SKU:
- PACO18210
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | MCM4 Antibody (PACO18210) |
Antibody SKU: | PACO18210 |
Size: | 50ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC |
Recommended Dilutions: | ELISA:1:1000-1:5000, WB:1:500-1:2000, IHC:1:50-1:200 |
Species Reactivity: | Human, Mouse |
Immunogen: | Synthetic peptide of human MCM4 |
Form: | Liquid |
Storage Buffer: | -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol |
Purification Method: | Antigen affinity purification |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using PACO18210(MCM4 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200). | |
Gel: 8%SDS-PAGE, Lysate: 50 μg, Lane 1-3: NIH/3T3 cells, Hela cells, K562 cells, Primary antibody: PACO18210(MCM4 Antibody) at dilution 1/700, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds. | |
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using PACO18210(MCM4 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200). |
Background: | The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported. |
Synonyms: | Minichromosome maintenance complex component 4 |
UniProt Protein Function: | MCM4: a mini-chromosome maintenance protein, essential for the initiation of eukaryotic genome replication. The MCM hexameric protein complex is a key component of the pre-replication complex. The complex consisting of this protein and MCM2, 6 and 7 possesses DNA helicase activity. Phosphorylation by CDC2 reduces the DNA helicase activity and chromatin binding of the complex. |
UniProt Protein Details: | Protein type:EC 3.6.4.12; DNA replication Chromosomal Location of Human Ortholog: 8q11.2 Cellular Component: MCM complex; membrane; nuclear chromosome, telomeric region; nucleoplasm Molecular Function:ATP-dependent DNA helicase activity; protein binding Biological Process: DNA replication; G1/S transition of mitotic cell cycle Disease: Natural Killer Cell And Glucocorticoid Deficiency With Dna Repair Defect |
NCBI Summary: | The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008] |
UniProt Code: | P33991 |
NCBI GenInfo Identifier: | 68571766 |
NCBI Gene ID: | 4173 |
NCBI Accession: | P33991.5 |
UniProt Secondary Accession: | P33991,Q8NEH1, Q99658, |
UniProt Related Accession: | P33991 |
Molecular Weight: | 96,558 Da |
NCBI Full Name: | DNA replication licensing factor MCM4 |
NCBI Synonym Full Names: | minichromosome maintenance complex component 4 |
NCBI Official Symbol: | MCM4 |
NCBI Official Synonym Symbols: | NKCD; CDC21; CDC54; NKGCD; hCdc21; P1-CDC21 |
NCBI Protein Information: | DNA replication licensing factor MCM4 |
UniProt Protein Name: | DNA replication licensing factor MCM4 |
UniProt Synonym Protein Names: | CDC21 homolog; P1-CDC21 |
Protein Family: | DNA replication licensing factor |
UniProt Gene Name: | MCM4 |
UniProt Entry Name: | MCM4_HUMAN |
Secondary Antibody |
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