MCM2 Antibody (PACO23682)
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주문- SKU:
- PACO23682
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | MCM2 Antibody (PACO23682) |
Antibody SKU: | PACO23682 |
Size: | 100ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:3000, IHC:1:50-1:100 |
Species Reactivity: | Human, Mouse, Rat |
Immunogen: | Synthesized peptide derived from human MCM2. |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MCM2 antibody. | |
Western blot analysis of extracts from 3T3 cells (Lane 2), k562 cells (Lane 3) and HepG2 cells (Lane 4), using MCM2 antiobdy. The lane on the left is treated with synthesized peptide. | |
Western blot analysis of extracts from 293 cells, using MCM2 antibody. |
Background: | Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. |
Synonyms: | BM28; CDCL1; DNA replication licensing factor MCM2; KIAA0030; MCMD2 |
UniProt Protein Function: | MCM2: a mini-chromosome maintenance protein, essential for the initiation of eukaryotic genome replication. Allows DNA to undergo a single round of replication per cell cycle. Required for the entry in S phase and for cell division. |
UniProt Protein Details: | Protein type:DNA-binding; EC 3.6.4.12 Chromosomal Location of Human Ortholog: 3q21 Cellular Component: microtubule cytoskeleton; nucleoplasm; MCM complex; cytoplasm; nuclear origin of replication recognition complex; nucleus; chromatin Molecular Function:protein binding; DNA helicase activity; DNA binding; histone binding; metal ion binding; DNA replication origin binding; ATP binding Biological Process: DNA unwinding during replication; nucleosome assembly; DNA replication initiation; DNA strand elongation during DNA replication; mitotic cell cycle; cell cycle; DNA replication; G1/S transition of mitotic cell cycle |
NCBI Summary: | The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Oct 2012] |
UniProt Code: | P49736 |
NCBI GenInfo Identifier: | 41019490 |
NCBI Gene ID: | 4171 |
NCBI Accession: | P49736.4 |
UniProt Secondary Accession: | P49736,Q14577, Q15023, Q8N2V1, Q969W7, Q96AE1, Q9BRM7 |
UniProt Related Accession: | P49736 |
Molecular Weight: | 904 |
NCBI Full Name: | DNA replication licensing factor MCM2 |
NCBI Synonym Full Names: | minichromosome maintenance complex component 2 |
NCBI Official Symbol: | MCM2 |
NCBI Official Synonym Symbols: | BM28; CCNL1; CDCL1; cdc19; D3S3194; MITOTIN |
NCBI Protein Information: | DNA replication licensing factor MCM2; cyclin-like 1; nuclear protein BM28; cell devision cycle-like 1; minichromosome maintenance protein 2 homolog; minichromosome maintenance deficient 2 (mitotin); MCM2 minichromosome maintenance deficient 2, mitotin |
UniProt Protein Name: | DNA replication licensing factor MCM2 |
UniProt Synonym Protein Names: | Minichromosome maintenance protein 2 homolog; Nuclear protein BM28 |
Protein Family: | DNA replication licensing factor |
UniProt Gene Name: | MCM2 |
UniProt Entry Name: | MCM2_HUMAN |
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