MASTL Antibody (PACO22800)
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주문- SKU:
- PACO22800
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | MASTL Antibody (PACO22800) |
Antibody SKU: | PACO22800 |
Size: | 100ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:3000 |
Species Reactivity: | Human |
Immunogen: | Synthesized peptide derived from C-terminal of human MASTL. |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western blot analysis of extracts from HeLa cells, using MASTL antibody. |
Background: | Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance. Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited. Phosphorylates histone protein in vitro; however such activity is unsure in vivo. May be involved in megakaryocyte differentiation. |
Synonyms: | FLJ14813; MASTL; microtubule associated serine/threonine kinase-like; Microtubule-associated serine/threonine-protein kinase-like; RP11-85G18.2 |
UniProt Protein Function: | MASTL: Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance. Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited. Phosphorylates histone protein in vitro; however such activity is unsure in vivo. May be involved in megakaryocyte differentiation. Defects in MASTL are the cause of thrombocytopenia type 2 (THC2). Thrombocytopenia is defined by a decrease in the number of platelets in circulating blood, resulting in the potential for increased bleeding and decreased ability for clotting. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. 3 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Protein kinase, AGC; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; AGC group; MAST family Chromosomal Location of Human Ortholog: 10p12.1 Cellular Component: nucleoplasm; centrosome; cytoplasm; nucleus; cleavage furrow Molecular Function:protein serine/threonine kinase activity; protein phosphatase 2A binding; kinase activity; ATP binding Biological Process: mitosis; regulation of cell cycle; cell division; mitotic cell cycle; G2/M transition of mitotic cell cycle; negative regulation of protein phosphatase type 2A activity; protein amino acid phosphorylation; response to DNA damage stimulus Disease: Thrombocytopenia 2 |
NCBI Summary: | This gene encodes a microtubule-associated serine/threonine kinase. Mutations at this locus have been associated with autosomal dominant thrombocytopenia, also known as thrombocytopenia-2. Alternatively spliced transcript variants have been described for this locus. [provided by RefSeq, Feb 2010] |
UniProt Code: | Q96GX5 |
NCBI GenInfo Identifier: | 288806587 |
NCBI Gene ID: | 84930 |
NCBI Accession: | NP_001165774.1 |
UniProt Secondary Accession: | Q96GX5,Q5T8D5, Q5T8D7, Q8NCD6, Q96SJ5, |
UniProt Related Accession: | Q96GX5 |
Molecular Weight: | 97,319 Da |
NCBI Full Name: | serine/threonine-protein kinase greatwall isoform 1 |
NCBI Synonym Full Names: | microtubule associated serine/threonine kinase-like |
NCBI Official Symbol: | MASTL |
NCBI Official Synonym Symbols: | GW; GWL; THC2; hGWL; MAST-L; GREATWALL; RP11-85G18.2 |
NCBI Protein Information: | serine/threonine-protein kinase greatwall; greatwall protein kinase; microtubule-associated serine/threonine-protein kinase-like |
UniProt Protein Name: | Serine/threonine-protein kinase greatwall |
UniProt Synonym Protein Names: | Microtubule-associated serine/threonine-protein kinase-like |
UniProt Gene Name: | MASTL |
UniProt Entry Name: | GWL_HUMAN |
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