MAPK1/MAPK3 (Ab-205/222) Antibody (PACO23087)
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주문- SKU:
- PACO23087
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
Antibody Name: | MAPK1/MAPK3 (Ab-205/222) Antibody (PACO23087) |
Antibody SKU: | PACO23087 |
Size: | 100ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:3000 |
Species Reactivity: | Human, Mouse, Rat |
Immunogen: | Synthesized peptide derived from Internal of human MAPK1. |
Form: | Liquid |
Storage Buffer: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western blot analysis of extracts from NIH-3T3 cells, using MAPK1/3 (Ab-205/222) antibody. | |
Western blot analysis of extracts from K562 cells (Lane 2), using MAPK1/3 (Ab-205/222) antiobdy. The lane on the left is treated with synthesized peptide. |
Background: | Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, DCC, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade. Mediates phosphorylation of TPR in respons to EGF stimulation. May play a role in the spindle assembly checkpoint. Phosphorylates PML and promotes its interaction with PIN1, leading to PML degradation. Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity. |
Synonyms: | ERK; ERK-2; ERK2; ERT1; Extracellular signal-regulated kinase 2 |
UniProt Protein Function: | ERK2: a serine/threonine kinase of the GMGC group that plays a critical role in the regulation of cell growth and differentiation. ERK1 (MAPK3) and ERK2 (MAPK1) play central roles in MAPK cascades and are activated by a wide variety of extracellular signals including growth and neurotrophic factors, cytokines, hormones and neurotransmitters. Depending on the cellular context, MAPK cascades mediate diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. MAPK cascades also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. Activation of MAP kinases occurs through phosphorylation of threonine and tyrosine residues at the sequence T*EY* by upstream MAP kinase kinases, MEK1 and -2. Phosphorylation of both the threonine and tyrosine are required for activity. This phosphorylation causes dramatic conformational changes, which enable full activation and interaction of MAPK1/ERK2 with its substrates. |
UniProt Protein Details: | Protein type:Protein kinase, CMGC; Kinase, protein; EC 2.7.11.24; Protein kinase, Ser/Thr (non-receptor); CMGC group; MAPK family; MAPK/ERK subfamily; ERK subfamily Chromosomal Location of Human Ortholog: 22q11.21 Cellular Component: axon; caveola; cytoplasm; cytoskeleton; cytosol; dendrite cytoplasm; early endosome; focal adhesion; Golgi apparatus; late endosome; microtubule cytoskeleton; microtubule organizing center; mitochondrion; nucleoplasm; nucleus; perikaryon; protein complex; pseudopodium Molecular Function:ATP binding; DNA binding; MAP kinase activity; mitogen-activated protein kinase kinase kinase binding; phosphatase binding; phosphotyrosine binding; protein binding; protein serine/threonine kinase activity; RNA polymerase subunit kinase activity; transcription factor binding Biological Process: activation of MAPK activity; activation of MAPKK activity; apoptosis; axon guidance; B cell receptor signaling pathway; Bergmann glial cell differentiation; blood coagulation; cell cycle; chemotaxis; cytosine metabolic process; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; innate immune response; insulin receptor signaling pathway; lipopolysaccharide-mediated signaling pathway; mammary gland epithelial cell proliferation; MAPKKK cascade; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; negative regulation of cell differentiation; nerve growth factor receptor signaling pathway; nuclear translocation of MAPK; outer ear morphogenesis; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; platelet activation; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of telomerase activity; positive regulation of telomere maintenance via telomerase; positive regulation of transcription, DNA-dependent; positive regulation of translation; protein amino acid phosphorylation; Ras protein signal transduction; regulation of cytoskeleton organization and biogenesis; regulation of protein stability; regulation of stress-activated MAPK cascade; regulation of transcription factor activity; response to DNA damage stimulus; response to estrogen stimulus; response to exogenous dsRNA; response to stress; response to toxin; sensory perception of pain; signal transduction; small GTPase mediated signal transduction; stress-activated MAPK cascade; synaptic transmission; T cell receptor signaling pathway; thymus development; thyroid gland development; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway; transcription, DNA-dependent; vascular endothelial growth factor receptor signaling pathway; viral reproduction |
NCBI Summary: | This gene encodes a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq, Jan 2014] |
UniProt Code: | P28482 |
NCBI GenInfo Identifier: | 119554 |
NCBI Gene ID: | 5594 |
NCBI Accession: | P28482.3 |
UniProt Secondary Accession: | P28482,A8CZ64, |
UniProt Related Accession: | P28482 |
Molecular Weight: | |
NCBI Full Name: | Mitogen-activated protein kinase 1 |
NCBI Synonym Full Names: | mitogen-activated protein kinase 1 |
NCBI Official Symbol: | MAPK1 |
NCBI Official Synonym Symbols: | ERK; p38; p40; p41; ERK2; ERT1; ERK-2; MAPK2; PRKM1; PRKM2; P42MAPK; p41mapk; p42-MAPK |
NCBI Protein Information: | mitogen-activated protein kinase 1 |
UniProt Protein Name: | Mitogen-activated protein kinase 1 |
UniProt Synonym Protein Names: | ERT1; Extracellular signal-regulated kinase 2; ERK-2; MAP kinase isoform p42; p42-MAPK; Mitogen-activated protein kinase 2; MAP kinase 2; MAPK 2 |
UniProt Gene Name: | MAPK1 |
UniProt Entry Name: | MK01_HUMAN |
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