MAGOH Antibody (PACO45603)
제품 코드를 사용하여 Assay Genie 메인 사이트를 통해 주문합니다.
주문- SKU:
- PACO45603
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IP
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | MAGOH Antibody (PACO45603) |
Antibody SKU: | PACO45603 |
Size: | 50ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC, IP |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
Species Reactivity: | Human, Mouse |
Immunogen: | Recombinant Human Protein mago nashi homolog protein (1-146AA) |
Form: | Liquid |
Storage Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Purification Method: | Antigen Affinity Purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western blot. All lanes: MAGOH antibody at 4.69µg/ml. Lane 1: Mouse kidney tissue. Lane 2: A431 whole cell lysate. Lane 3: Jurkat whole cell lysate. Lane 4: Raji whole cell lysate. Lane 5: K562 whole cell lysate. Lane 6: Hela whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 18, 13 kDa. Observed band size: 18 kDa.. | |
Immunohistochemistry of paraffin-embedded human kidney tissue using PACO45603 at dilution of 1:100. | |
Immunohistochemistry of paraffin-embedded human ovarian cancer using PACO45603 at dilution of 1:100. |
Background: | Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The MAGOH-RBM8A heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The MAGOH-RBM8A heterodimer interacts with the EJC key regulator PYM1 leading to EJC disassembly in the cytoplasm and translation enhancement of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the function is different from the established EJC assembly. |
Synonyms: | Protein mago nashi homolog, MAGOH, MAGOHA |
UniProt Protein Function: | MAGOH: Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Remains associated with the mRNA after its export to the cytoplasm and require translation of the mRNA for removal. The heterodimer MAGOH-RBM8A interacts with PYM that function to enhance the translation of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Belongs to the mago nashi family. |
UniProt Protein Details: | Protein type:RNA splicing; Spliceosome Chromosomal Location of Human Ortholog: 1p32.3 Cellular Component: nucleoplasm; nuclear speck; nucleus; cytosol Molecular Function:protein binding Biological Process: regulation of translation; transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; mRNA export from nucleus; RNA splicing; mRNA catabolic process, nonsense-mediated decay; gene expression; mRNA 3'-end processing; termination of RNA polymerase II transcription; regulation of alternative nuclear mRNA splicing, via spliceosome |
NCBI Summary: | Drosophila that have mutations in their mago nashi (grandchildless) gene produce progeny with defects in germplasm assembly and germline development. This gene encodes the mammalian mago nashi homolog. In mammals, mRNA expression is not limited to the germ plasm, but is expressed ubiquitously in adult tissues and can be induced by serum stimulation of quiescent fibroblasts. [provided by RefSeq, Jul 2008] |
UniProt Code: | P61326 |
NCBI GenInfo Identifier: | 47117708 |
NCBI Gene ID: | 4116 |
NCBI Accession: | P61326.1 |
UniProt Secondary Accession: | P61326,O35169, P50606, Q5SW69, B1ARP8, B2R5A2, |
UniProt Related Accession: | P61326 |
Molecular Weight: | 146 |
NCBI Full Name: | Protein mago nashi homolog |
NCBI Synonym Full Names: | mago-nashi homolog, proliferation-associated (Drosophila) |
NCBI Official Symbol: | MAGOH |
NCBI Official Synonym Symbols: | MAGOH1; MAGOHA |
NCBI Protein Information: | protein mago nashi homolog |
UniProt Protein Name: | Protein mago nashi homolog |
Protein Family: | Protein mago nashi |
UniProt Gene Name: | MAGOH |
UniProt Entry Name: | MGN_HUMAN |