LIN28B Antibody (PACO21520)
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주문- SKU:
- PACO21520
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | LIN28B Antibody (PACO21520) |
Antibody SKU: | PACO21520 |
Size: | 100ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:1000 |
Species Reactivity: | Human |
Immunogen: | Peptide sequence around aa.242~246( P-S-V-Q-K) derived from Human LIN28B |
Form: | Liquid |
Storage Buffer: | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Purification Method: | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates Heo I., Joo C., Kim YK., Kim V.N.,et al. Cell 138:696-708(2009) " |
Clonality: | Polyclonal |
Conjugate: | Non-conjugated |
Western blot analysis of extracts from Hela and 293 cells using LIN28B Antibody. |
Synonyms: | CSDD2; FLJ16517; |
UniProt Protein Function: | LIN28B: Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre- let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth. Interacts with ZCCHC11/TUT4. Might be negatively regulated by the microRNA let-7b. High expression in testis, fetal liver, placenta and in hepatocellular carcinoma (HCC). Isoform 1 is only detected in moderately and poorly differentiated HCC tissues and placenta. Isoform 2 is detected in fetal liver, non-tumor liver tissues, as well as well-differentiated tumor tissues. Belongs to the lin-28 family. 2 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Unknown function Chromosomal Location of Human Ortholog: 6q21 Molecular Function:protein binding; RNA binding Biological Process: pre-microRNA processing; RNA 3'-end processing |
NCBI Summary: | The protein encoded by this gene belongs to the lin-28 family, which is characterized by the presence of a cold-shock domain and a pair of CCHC zinc finger domains. This gene is highly expressed in testis, fetal liver, placenta, and in primary human tumors and cancer cell lines. It is negatively regulated by microRNAs that target sites in the 3' UTR, and overexpression of this gene in primary tumors is linked to the repression of let-7 family of microRNAs and derepression of let-7 targets, which facilitates cellular transformation. [provided by RefSeq, Jun 2012] |
UniProt Code: | Q6ZN17 |
NCBI GenInfo Identifier: | 74758701 |
NCBI Gene ID: | 389421 |
NCBI Accession: | Q6ZN17.1 |
UniProt Secondary Accession: | Q6ZN17,Q5TCM4, A1L165, B2RPN6, |
UniProt Related Accession: | Q6ZN17 |
Molecular Weight: | 19,511 Da |
NCBI Full Name: | Protein lin-28 homolog B |
NCBI Synonym Full Names: | lin-28 homolog B |
NCBI Official Symbol: | LIN28B |
NCBI Official Synonym Symbols: | CSDD2 |
NCBI Protein Information: | protein lin-28 homolog B |
UniProt Protein Name: | Protein lin-28 homolog B |
Protein Family: | Protein |
UniProt Gene Name: | LIN28B |
UniProt Entry Name: | LN28B_HUMAN |