LIME1 Antibody (PACO61682)
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주문- SKU:
- PACO61682
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | LIME1 Antibody (PACO61682) |
Antibody SKU: | PACO61682 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:500-1:1000, IF:1:50-1:200 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Lck-interacting transmembrane adapter 1 protein (170-295AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
IHC image of PACO61682 diluted at 1:500 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
Immunofluorescence staining of HepG2 cells with PACO61682 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). | |
IHC image of PACO61682 diluted at 1:500 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
Background: | Involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and TCR (T-cell antigen receptor)-mediated T-cell signaling in T-cells. In absence of TCR signaling, may be involved in CD4-mediated inhibition of T-cell activation. Couples activation of these receptors and their associated kinases with distal intracellular events such as calcium mobilization or MAPK activation through the recruitment of PLCG2, GRB2, GRAP2, and other signaling molecules. |
Synonyms: | Lck-interacting transmembrane adapter 1, Lck-interacting membrane protein, Lck-interacting molecule, LIME1, LIME, LP8067 |
UniProt Protein Function: | LIME1: Involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and TCR (T-cell antigen receptor)-mediated T- cell signaling in T-cells. In absence of TCR signaling, may be involved in CD4-mediated inhibition of T-cell activation. Couples activation of these receptors and their associated kinases with distal intracellular events such as calcium mobilization or MAPK activation through the recruitment of PLCG2, GRB2, GRAP2, and other signaling molecules. 2 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Membrane protein, integral; Adaptor/scaffold Chromosomal Location of Human Ortholog: 20q13.3 Cellular Component: extracellular space |
NCBI Summary: | This gene encodes a transmembrane adaptor protein that links the T and B-cell receptor stimulation to downstream signaling pathways via its association with the Src family kinases Lck and Lyn, respectively. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Mar 2015] |
UniProt Code: | Q9H400 |
NCBI GenInfo Identifier: | 74752630 |
NCBI Gene ID: | 54923 |
NCBI Accession: | Q9H400.1 |
UniProt Secondary Accession: | Q9H400,Q5JWJ2, Q6XYB3, Q9NX69, E1P5K5, E1P5K6, |
UniProt Related Accession: | Q9H400 |
Molecular Weight: | 20,841 Da |
NCBI Full Name: | Lck-interacting transmembrane adapter 1 |
NCBI Synonym Full Names: | Lck interacting transmembrane adaptor 1 |
NCBI Official Symbol: | LIME1 |
NCBI Official Synonym Symbols: | LIME; dJ583P15.4 |
NCBI Protein Information: | lck-interacting transmembrane adapter 1 |
UniProt Protein Name: | Lck-interacting transmembrane adapter 1 |
UniProt Synonym Protein Names: | Lck-interacting molecule |
Protein Family: | Lck-interacting transmembrane adapter |
UniProt Gene Name: | LIME1 |
UniProt Entry Name: | LIME1_HUMAN |
Antibodies |
Anti-LIME1 Antibody (CAB13719) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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