IDH2 Antibody (PACO44084)
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주문- SKU:
- PACO44084
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | IDH2 Antibody (PACO44084) |
Antibody SKU: | PACO44084 |
Size: | 50ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:2000 |
Species Reactivity: | Human, Mouse |
Immunogen: | Recombinant Human Isocitrate dehydrogenase [NADP], mitochondrial protein (193-452AA) |
Form: | Liquid |
Storage Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Purification Method: | Antigen Affinity Purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western blot. All lanes: IDH2 antibody at 5µg/ml. Lane 1: Hela whole cell lysate. Lane 2: Mouse heart tissue. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 51, 46 kDa. Observed band size: 51 kDa.. |
Background: | Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex. |
Synonyms: | Isocitrate dehydrogenase [NADP], mitochondrial (IDH) (EC 1.1.1.42) (ICD-M) (IDP) (NADP(+)-specific ICDH) (Oxalosuccinate decarboxylase), IDH2 |
UniProt Protein Function: | IDH2: a mitochondrial oxidoreductase that catalyzes the third step of the citric acid cycle: the oxidative decarboxylation of isocitrate, consuming NADP(+), and producing alpha-ketoglutarate (alpha-KG) and CO2. Can also use oxalosuccinate as a substrate. Alpha-KG is an activator the dioxygenases that hydroxylate the transcription factor HIF and lead to its degradation by VHL. Since HIF turns on oncogenic pathways, IDH2 has apparent tumor suppressor activity. Homo-dimerization is required for activity. Each subunit binds 1 magnesium or manganese ion. May tightly associate or interact with the pyruvate dehydrogenase complex. A somatic mutation resulting in the R172K conversion results in a neomorphic enzyme activity that converts isocitrate to R(-)-2-hydroxyglutarate (2HG) occurs in a significant percentage of patients with cytogenetically normal acute myeloid leukemia (AML). Elevated levels of 2HG are also correlated with an elevated risk of malignant brain tumors. |
UniProt Protein Details: | Protein type:Mitochondrial; Carbohydrate Metabolism - citrate (TCA) cycle; EC 1.1.1.42; Other Amino Acids Metabolism - glutathione; Oxidoreductase Chromosomal Location of Human Ortholog: 15q26.1 Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial inner membrane Molecular Function:magnesium ion binding; isocitrate dehydrogenase (NADP+) activity; NAD binding Biological Process: glyoxylate cycle; isocitrate metabolic process; cellular metabolic process; tricarboxylic acid cycle; carbohydrate metabolic process; 2-oxoglutarate metabolic process Disease: D-2-hydroxyglutaric Aciduria 2 |
NCBI Summary: | Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014] |
UniProt Code: | P48735 |
NCBI GenInfo Identifier: | 20141568 |
NCBI Gene ID: | 3418 |
NCBI Accession: | P48735.2 |
UniProt Secondary Accession: | P48735,Q96GT3, B2R6L6, B4DFL2, |
UniProt Related Accession: | P48735 |
Molecular Weight: | 452 |
NCBI Full Name: | Isocitrate dehydrogenase |
NCBI Synonym Full Names: | isocitrate dehydrogenase 2 (NADP+), mitochondrial |
NCBI Official Symbol: | IDH2 |
NCBI Official Synonym Symbols: | IDH; IDP; IDHM; IDPM; ICD-M; D2HGA2; mNADP-IDH |
NCBI Protein Information: | isocitrate dehydrogenase [NADP], mitochondrial; isocitrate dehydrogenase [NADP], mitochondrial; NADP(+)-specific ICDH; oxalosuccinate decarboxylase |
UniProt Protein Name: | Isocitrate dehydrogenase [NADP], mitochondrial |
UniProt Synonym Protein Names: | ICD-M; IDP; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase |
Protein Family: | Isocitrate dehydrogenase |
UniProt Gene Name: | IDH2 |
UniProt Entry Name: | IDHP_HUMAN |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |