Protein Kinase C Epsilon type is a member of the serine- and threonine-specific protein kinase family that can be activated by calcium and the second messenger diacylglycerol. Protein Kinase C Epsilon contains these domains: one AGC-kinase C-terminal domain; one C2 domain; one protein kinase domain and two phorbol-ester/DAG-type zinc fingers. Protein Kinase C Epsilon phosphorylate a variety of protein targets and has been identified to participate in diverse cellular signaling pathways. It has many different cellular functions; such as neuron channel activation; apoptosis; cardioprotection from ischemia; heat shock response; as well as insulin exocytosis. Protein Kinase C Epsilon also serves as the receptor for phorbol esters; a class of tumor promoters.
Product Name:
Human PKC epsilon/PKCE Recombinant Protein (RPES3223)
Product Code:
RPES3223
Size:
10µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
Protein Kinase C Epsilon Type, nPKC-Epsilon, PRKCE, PKCE
Accession:
Q02156
Sequence:
Gln580-Pro737
Fusion tag:
C-6His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human PKC epsilon is produced by our E.coli expression system and the target gene encoding Gln580-Pro737 is expressed with a 6His tag at the C-terminus.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
19.6 kDa
AP Mol Mass:
20 kDa
Formulation:
Supplied as a 0.2 µm filtered solution of 20mM PB; 150mM NaCl; 1mM DTT; pH 7.4.
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt; store it immediately at<-20°C.
Stability and Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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