CD86; also known as B-lymphocyte activation antigen B7-2 (referred to as B70); is a member of the cell surface immunoglobulin superfamily. B7-2 exists predominantly as a monomer on cell surfaces and interacts with two co-stimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells; and thus induces the signal pathways which regulate T cell activation and tolerance; cytokine production; and the generation of CTL. It is indicated that contacts between B and T helper cells mediated by CD86 encourage signals for the proliferation and IgG secretion of normal B cells and B cell lymphomas. Recent study has revealed that CD86 also promotes the generation of a mature APC repertoire and promotes APC function and survival. CD86 has an important role in chronic hemodialysis; allergic pulmonary inflammation; arthritis; and antiviral responses; and thus is regarded as a promising candidate for immune therapy.Immune CheckpointImmune Checkpoint Detection: Antibodies Immune Checkpoint Detection: ELISA Antibodies Immune Checkpoint Detection: IP Antibodies Immune Checkpoint Detection: FCM Antibodies Immune Checkpoint Detection: WB AntibodiesImmune Checkpoint ProteinsImmune Checkpoint Targets Co-inhibitory Immune Checkpoint Targets Immunotherapy Cancer Immunotherapy Targeted Therapy
1. Measured by its ability to bind human CD28 in a functional ELISA.2. Measured by its ability to induce IL2 secretion by Jurkat human acute T cell leukemia cells.The ED50 for this effect is 1-5µg/mL.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the extracellular domain (Met 1-His 239) of human B7-2 (NP_008820.2) pro-protein was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
53.2 kDa
AP Mol Mass:
80-90 kDa
Formulation:
Lyophilized from sterile PBS; pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
system_update_alt데이터시트Mouse CD86/B7-2 Recombinant Protein T-lymphocyte activation antigen CD86 (B7-2) is a glycosylated protein in the B7 family. B7 family members are transmembrane cell surface
system_update_alt데이터시트Cynomolgus B7-2/CD86 Recombinant Protein T-lymphocyte activation antigen CD86 (B7-2) is a glycosylated protein in the B7 family. B7 family members are transmembrane cell sur
system_update_alt데이터시트Mouse CD86/B7-2 Recombinant Protein CD86, also known as B-lymphocyte activation antigen B7-2 (referred to as B70), is a member of the cell surface immunoglobulin superfamily
system_update_alt데이터시트Human B7-H4/VTCN1 Recombinant Protein V-set domain-containing T-cell activation inhibitor 1, also known as B7X, B7H4, B7S1, and VTCN1, is a single-pass type? membrane protei
system_update_alt데이터시트Human B7-DC/PD-L2/CD273 Recombinant Protein Programmed death ligand 2 (PD-L2); also referred to as B7-DC and CD273; is a member of the B7 family of proteins including B7-1;
