HSD11B2 Antibody (PACO55546)
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주문- SKU:
- PACO55546
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | HSD11B2 Antibody (PACO55546) |
Antibody SKU: | PACO55546 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Corticosteroid 11-beta-dehydrogenase isozyme 2 protein (212-343AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: 293 whole cell lysate. All lanes: HSD11B2 antibody at 2.4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 45 kDa. Observed band size: 45 kDa. | |
IHC image of PACO55546 diluted at 1:200 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
Background: | Catalyzes the conversion of cortisol to the inactive metabolite cortisone. Modulates intracellular glucocorticoid levels, thus protecting the nonselective mineralocorticoid receptor from occupation by glucocorticoids. |
Synonyms: | Corticosteroid 11-beta-dehydrogenase isozyme 2 (EC 1.1.1) (11-beta-hydroxysteroid dehydrogenase type 2) (11-DH2) (11-beta-HSD2) (11-beta-hydroxysteroid dehydrogenase type II) (11-HSD type II) (11-beta-HSD type II) (NAD-dependent 11-beta-hydroxysteroid dehydrogenase) (11-beta-HSD) (Short chain dehydrogenase/reductase family 9C member 3), HSD11B2, HSD11K SDR9C3 |
UniProt Protein Function: | HSD11B2: Catalyzes the conversion of cortisol to the inactive metabolite cortisone. Modulates intracellular glucocorticoid levels, thus protecting the nonselective mineralocorticoid receptor from occupation by glucocorticoids. Defects in HSD11B2 are the cause of apparent mineralocorticoid excess (AME). An autosomal recessive form of low-renin hypertension. It is usually diagnosed within the first years of life and is characterized by polyuria and polydipsia, failure to thrive, hypernatremia, severe hypertension with low renin and aldosterone levels, profound hypokalemia with metabolic alkalosis, and most often nephrocalcinosis. Belongs to the short-chain dehydrogenases/reductases (SDR) family. |
UniProt Protein Details: | Protein type:Lipid Metabolism - androgen and estrogen; Oxidoreductase; EC 1.1.1.-; Lipid Metabolism - C21-steroid hormone Chromosomal Location of Human Ortholog: 16q22 Cellular Component: endoplasmic reticulum membrane Molecular Function:11-beta-hydroxysteroid dehydrogenase activity Biological Process: glucocorticoid biosynthetic process Disease: Apparent Mineralocorticoid Excess |
NCBI Summary: | There are at least two isozymes of the corticosteroid 11-beta-dehydrogenase, a microsomal enzyme complex responsible for the interconversion of cortisol and cortisone. The type I isozyme has both 11-beta-dehydrogenase (cortisol to cortisone) and 11-oxoreductase (cortisone to cortisol) activities. The type II isozyme, encoded by this gene, has only 11-beta-dehydrogenase activity. In aldosterone-selective epithelial tissues such as the kidney, the type II isozyme catalyzes the glucocorticoid cortisol to the inactive metabolite cortisone, thus preventing illicit activation of the mineralocorticoid receptor. In tissues that do not express the mineralocorticoid receptor, such as the placenta and testis, it protects cells from the growth-inhibiting and/or pro-apoptotic effects of cortisol, particularly during embryonic development. Mutations in this gene cause the syndrome of apparent mineralocorticoid excess and hypertension. [provided by RefSeq, Feb 2010] |
UniProt Code: | P80365 |
NCBI GenInfo Identifier: | 30316367 |
NCBI Gene ID: | 3291 |
NCBI Accession: | P80365.2 |
UniProt Secondary Accession: | P80365,Q13194, Q6P2G9, Q8N439, Q96QN8, Q9UC50, Q9UC51 Q9UCW5, Q9UCW6, Q9UCW7, A7LB28, C5HTY7, |
UniProt Related Accession: | P80365 |
Molecular Weight: | 44,127 Da |
NCBI Full Name: | Corticosteroid 11-beta-dehydrogenase isozyme 2 |
NCBI Synonym Full Names: | hydroxysteroid 11-beta dehydrogenase 2 |
NCBI Official Symbol: | HSD11B2 |
NCBI Official Synonym Symbols: | AME; AME1; HSD2; HSD11K; SDR9C3 |
NCBI Protein Information: | corticosteroid 11-beta-dehydrogenase isozyme 2 |
UniProt Protein Name: | Corticosteroid 11-beta-dehydrogenase isozyme 2 |
UniProt Synonym Protein Names: | 11-beta-hydroxysteroid dehydrogenase type 2; 11-DH2; 11-beta-HSD2; 11-beta-hydroxysteroid dehydrogenase type II; 11-HSD type II; 11-beta-HSD type II; NAD-dependent 11-beta-hydroxysteroid dehydrogenase; 11-beta-HSD; Short chain dehydrogenase/reductase family 9C member 3 |
Protein Family: | Corticosteroid 11-beta-dehydrogenase isozyme |
UniProt Gene Name: | HSD11B2 |
UniProt Entry Name: | DHI2_HUMAN |