HSD11B1 Antibody (PACO27457)
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주문- SKU:
- PACO27457
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | HSD11B1 Antibody (PACO27457) |
Antibody SKU: | PACO27457 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Corticosteroid 11-beta-dehydrogenase isozyme 1 protein (25-292AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western blot All lanes: HSD11B1 antibody at 2µg/ml + Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 33 kDa Observed band size: 33 kDa . | |
Immunofluorescent analysis of Hela cells using PACO27457 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). | |
Immunohistochemistry of paraffin-embedded human adrenal gland tissue using PACO27457 at dilution of 1:100. |
Background: | Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone. Catalyzes reversibly the conversion of 7-ketocholesterol to 7-beta-hydroxycholesterol. In intact cells, the reaction runs only in one direction, from 7-ketocholesterol to 7-beta-hydroxycholesterol. |
Synonyms: | Corticosteroid 11-beta-dehydrogenase isozyme 1 (EC 1.1.1.146) (11-beta-hydroxysteroid dehydrogenase 1) (11-DH) (11-beta-HSD1) (Short chain dehydrogenase/reductase family 26C member 1), HSD11B1, HSD11 HSD11L SDR26C1 |
UniProt Protein Function: | HSD11B1: Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone. Catalyzes reversibly the conversion of 7-ketocholesterol to 7-beta-hydroxycholesterol. In intact cells, the reaction runs only in one direction, from 7- ketocholesterol to 7-beta-hydroxycholesterol. Defects in HSD11B1 are a cause of cortisone reductase deficiency (CRD). In CRD, activation of cortisone to cortisol does not occur, resulting in adrenocorticotropin-mediated androgen excess and a phenotype resembling polycystic ovary syndrome (PCOS). Belongs to the short-chain dehydrogenases/reductases (SDR) family. |
UniProt Protein Details: | Protein type:Lipid Metabolism - androgen and estrogen; Lipid Metabolism - C21-steroid hormone; Endoplasmic reticulum; EC 1.1.1.146; Membrane protein, integral; Oxidoreductase Chromosomal Location of Human Ortholog: 1q32-q41 Cellular Component: endoplasmic reticulum membrane; membrane; integral to membrane Molecular Function:11-beta-hydroxysteroid dehydrogenase activity Biological Process: steroid metabolic process; glucocorticoid biosynthetic process; lung development Disease: Cortisone Reductase Deficiency 2 |
NCBI Summary: | The protein encoded by this gene is a microsomal enzyme that catalyzes the conversion of the stress hormone cortisol to the inactive metabolite cortisone. In addition, the encoded protein can catalyze the reverse reaction, the conversion of cortisone to cortisol. Too much cortisol can lead to central obesity, and a particular variation in this gene has been associated with obesity and insulin resistance in children. Mutations in this gene and H6PD (hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase)) are the cause of cortisone reductase deficiency. Alternate splicing results in multiple transcript variants encoding the same protein.[provided by RefSeq, May 2011] |
UniProt Code: | P28845 |
NCBI GenInfo Identifier: | 118569 |
NCBI Gene ID: | 3290 |
NCBI Accession: | P28845.3 |
UniProt Secondary Accession: | P28845,B2R9Z1, D3DT89, |
UniProt Related Accession: | P28845 |
Molecular Weight: | |
NCBI Full Name: | Corticosteroid 11-beta-dehydrogenase isozyme 1 |
NCBI Synonym Full Names: | hydroxysteroid (11-beta) dehydrogenase 1 |
NCBI Official Symbol: | HSD11B1 |
NCBI Official Synonym Symbols: | HDL; 11-DH; HSD11; HSD11B; HSD11L; CORTRD2; SDR26C1; 11-beta-HSD1 |
NCBI Protein Information: | corticosteroid 11-beta-dehydrogenase isozyme 1; short chain dehydrogenase/reductase family 26C, member 1 |
UniProt Protein Name: | Corticosteroid 11-beta-dehydrogenase isozyme 1 |
UniProt Synonym Protein Names: | 11-beta-hydroxysteroid dehydrogenase 1; 11-DH; 11-beta-HSD1 |
Protein Family: | Corticosteroid 11-beta-dehydrogenase isozyme |
UniProt Gene Name: | HSD11B1 |
UniProt Entry Name: | DHI1_HUMAN |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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