HPN Antibody (PACO57812)
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주문- SKU:
- PACO57812
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
Antibody Name: | HPN Antibody (PACO57812) |
Antibody SKU: | PACO57812 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200 |
Species Reactivity: | Human, Mouse, Rat |
Immunogen: | Recombinant Human Serine protease hepsin protein (294-413AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: MCF-7 whole cell lysate, PC-3 whole cell lysate, Rat kidney tissue, Mouse liver tissue. All lanes: HPN antibody at 4.5µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 46 kDa. Observed band size: 46 kDa. | |
Immunofluorescence staining of MCF-7 cells with PACO57812 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). | |
IHC image of PACO57812 diluted at 1:400 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
Background: | Serine protease that cleaves extracellular substrates, and contributes to the proteolytic processing of growth factors, such as HGF and MST1/HGFL. Plays a role in cell growth and maintenance of cell morphology. Plays a role in the proteolytic processing of ACE2. Mediates the proteolytic cleavage of urinary UMOD that is required for UMOD polymerization. |
Synonyms: | Serine protease hepsin (EC 3.4.21.106) (Transmembrane protease serine 1) [Cleaved into: Serine protease hepsin non-catalytic chain; Serine protease hepsin catalytic chain], HPN, TMPRSS1 |
UniProt Protein Function: | HPN: Plays an essential role in cell growth and maintenance of cell morphology. Belongs to the peptidase S1 family. |
UniProt Protein Details: | Protein type:Membrane protein, integral; EC 3.4.21.106; Protease Chromosomal Location of Human Ortholog: 19q13.12 Cellular Component: endoplasmic reticulum membrane; nuclear membrane; cell surface; cell soma; integral to plasma membrane; plasma membrane; integral to membrane; intercellular junction Molecular Function:peptidase activity; calcium-activated potassium channel activity; protein binding; serine-type peptidase activity; serine-type endopeptidase activity Biological Process: regulation of cell shape; cholesterol homeostasis; detection of mechanical stimulus involved in sensory perception of sound; proteolysis; positive regulation of cell growth; negative regulation of epithelial cell proliferation; negative regulation of apoptosis |
NCBI Summary: | This gene encodes a type II transmembrane serine protease that may be involved in diverse cellular functions, including blood coagulation and the maintenance of cell morphology. Expression of the encoded protein is associated with the growth and progression of cancers, particularly prostate cancer. The protein is cleaved into a catalytic serine protease chain and a non-catalytic scavenger receptor cysteine-rich chain, which associate via a single disulfide bond. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013] |
UniProt Code: | P05981 |
NCBI GenInfo Identifier: | 123057 |
NCBI Gene ID: | 3249 |
NCBI Accession: | P05981.1 |
UniProt Related Accession: | P05981 |
Molecular Weight: | 45 kDa |
NCBI Full Name: | Serine protease hepsin |
NCBI Synonym Full Names: | hepsin |
NCBI Official Symbol: | HPN |
NCBI Official Synonym Symbols: | TMPRSS1 |
NCBI Protein Information: | serine protease hepsin |
UniProt Protein Name: | Serine protease hepsin |
UniProt Synonym Protein Names: | Transmembrane protease serine 1Cleaved into the following 2 chains:Serine protease hepsin non-catalytic chain; Serine protease hepsin catalytic chain |
Protein Family: | Histidine-rich metal-binding polypeptide |
UniProt Gene Name: | HPN |
UniProt Entry Name: | HEPS_HUMAN |
Antibodies | ELISA Kits |
HPN Antibody (PACO09787) | Human Hepsin ELISA Kit |
Human Serine protease hepsin (HPN) ELISA Kit |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
Recommended Products |
Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |