HIF3A Antibody (PACO52922)
제품 코드를 사용하여 Assay Genie 메인 사이트를 통해 주문합니다.
주문- SKU:
- PACO52922
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | HIF3A Antibody (PACO52922) |
Antibody SKU: | PACO52922 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:500-1:1000 |
Species Reactivity: | Human, Mouse |
Immunogen: | Recombinant Human Hypoxia-inducible factor 3-α protein (516-669AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate, HEK293 whole cell lysate, Mouse heart tissue. All lanes: HIF3A antibody at 2.5µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 73, 69, 40, 67, 63, 65 kDa. Observed band size: 73, 69 kDa. | |
IHC image of PACO52922 diluted at 1:500 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
Background: | Acts as a transcriptional regulator in adaptive response to low oxygen tension. Acts as a regulator of hypoxia-inducible gene expression. Functions as an inhibitor of angiogenesis in hypoxic cells of the cornea. Plays a role in the development of the cardiorespiratory system. May also be involved in apoptosis (By similarity). |
Synonyms: | Hypoxia-inducible factor 3-α (HIF-3-alpha) (HIF3-alpha) (Basic-helix-loop-helix-PAS protein MOP7) (Class E basic helix-loop-helix protein 17) (bHLHe17) (HIF3-alpha-1) (Inhibitory PAS domain protein) (IPAS) (Member of PAS protein 7) (PAS domain-containing protein 7), HIF3A, BHLHE17 MOP7 PASD7 |
UniProt Protein Function: | HIF3A: Involved in adaptive response to hypoxia. Suppresses hypoxia-inducible expression of HIF1A and EPAS1. Binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. The complex HIF3A-ARNT activates the transcription of reporter genes driven by HRE. Isoform 4 has a dominant-negative function of inactivating HIF1A-mediated transcription. Isoform 4 attenuates the binding of HIF1A to hypoxia-responsive elements (HRE), thus inhibiting HRE-driven transcription. Hypoxia induces down-regulation of isoform 4, leading to activation of HIF1A in hypoxia. Conversely, upon restoring normoxia, the expression of isoform 4 increases and thereby secure an inhibition of HIF1A activity. Isoform 4 may be a negative regulator of hypoxia-inducible gene expression in the kidney and may be involved in renal tumorigenesis. Functions as an inhibitor of angiogenesis in the cornea. 7 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Transcription factor; Tumor suppressor Chromosomal Location of Human Ortholog: 19q13.32 Cellular Component: nucleoplasm; cytosol Molecular Function:protein dimerization activity; signal transducer activity; DNA binding; transcription coactivator activity; transcription corepressor activity; transcription factor activity Biological Process: regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; signal transduction |
NCBI Summary: | The protein encoded by this gene is the alpha-3 subunit of one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia). The alpha-3 subunit lacks the transactivation domain found in factors containing either the alpha-1 or alpha-2 subunits. It is thought that factors containing the alpha-3 subunit are negative regulators of hypoxia-inducible gene expression. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2011] |
UniProt Code: | Q9Y2N7 |
NCBI GenInfo Identifier: | 145558932 |
NCBI Gene ID: | 64344 |
NCBI Accession: | Q9Y2N7.2 |
UniProt Secondary Accession: | Q9Y2N7,Q58A43, Q66K72, Q8WXA1, Q96K34, Q9H7Z9, Q9HAI2 B0M185, B4DNA2, |
UniProt Related Accession: | Q9Y2N7 |
Molecular Weight: | 64,359 Da |
NCBI Full Name: | Hypoxia-inducible factor 3-alpha |
NCBI Synonym Full Names: | hypoxia inducible factor 3, alpha subunit |
NCBI Official Symbol: | HIF3A |
NCBI Official Synonym Symbols: | IPAS; MOP7; PASD7; HIF-3A; bHLHe17 |
NCBI Protein Information: | hypoxia-inducible factor 3-alpha; HIF3-alpha-1; member of PAS protein 7; inhibitory PAS domain protein; PAS domain-containing protein 7; basic-helix-loop-helix-PAS protein MOP7; class E basic helix-loop-helix protein 17 |
UniProt Protein Name: | Hypoxia-inducible factor 3-alpha |
UniProt Synonym Protein Names: | Basic-helix-loop-helix-PAS protein MOP7; Class E basic helix-loop-helix protein 17; bHLHe17; HIF3-alpha-1; Inhibitory PAS domain protein; IPAS; Member of PAS protein 7; PAS domain-containing protein 7 |
Protein Family: | Hypoxia-inducible factor |
UniProt Gene Name: | HIF3A |
UniProt Entry Name: | HIF3A_HUMAN |
Antibodies |
Anti-HIF3A Antibody (CAB20020) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
Recommended Products |
Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |