HERC5 Antibody (PACO59473)
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주문- SKU:
- PACO59473
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | HERC5 Antibody (PACO59473) |
Antibody SKU: | PACO59473 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:20-1:200, IF:1:20-1:200 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human E3 ISG15--protein ligase HERC5 protein (153-284AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
IHC image of PACO59473 diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
Immunofluorescence staining of Hela cells with PACO59473 at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
Background: | Major E3 ligase for ISG15 conjugation. Acts as a positive regulator of innate antiviral response in cells induced by interferon. Functions as part of the ISGylation machinery that recognizes target proteins in a broad and relatively non-specific manner. Catalyzes ISGylation of IRF3 which results in sustained activation, it attenuates IRF3-PIN1 interaction, which antagonizes IRF3 ubiquitination and degradation, and boosts the antiviral response. Catalyzes ISGylation of influenza A viral NS1 which attenuates virulence; ISGylated NS1 fails to form homodimers and thus to interact with its RNA targets. Catalyzes ISGylation of papillomavirus type 16 L1 protein which results in dominant-negative effect on virus infectivity. Physically associated with polyribosomes, broadly modifies newly synthesized proteins in a cotranslational manner. In an interferon-stimulated cell, newly translated viral proteins are primary targets of ISG15. |
Synonyms: | E3 ISG15--protein ligase HERC5 (EC 2.3.2) (Cyclin-E-binding protein 1) (HECT domain and RCC1-like domain-containing protein 5), HERC5, CEB1 CEBP1 |
UniProt Protein Function: | HERC5: Major E3 ligase for ISG15 conjugation. Acts as a positive regulator of innate antiviral response in cells induced by interferon. Makes part of the ISGylation machinery that recognizes target proteins in a broad and relatively non-specific manner. Catalyzes ISGylation of IRF3 which results in sustained activation, it attenuates IRF3-PIN1 interaction, which antagonizes IRF3 ubiquitination and degradation, and boosts the antiviral response. Catalyzes ISGylation of influenza A viral NS1 which attenuates virulence; ISGylated NS1 fails to form homodimers and thus to interact with its RNA targets. Catalyzes ISGylation of papillomavirus type 16 L1 protein which results in dominant- negative effect on virus infectivity. Physically associated with polyribosomes, broadly modifies newly synthesized proteins in a cotranslational manner. In an interferon-stimulated cell, newly translated viral proteins are primary targets of ISG15. |
UniProt Protein Details: | Protein type:Ligase; EC 6.3.2.-; Ubiquitin conjugating system; Ubiquitin ligase Chromosomal Location of Human Ortholog: 4q22.1 Cellular Component: cytosol; perinuclear region of cytoplasm Molecular Function:ISG15 conjugating enzyme activity; ligase activity; protein binding; ubiquitin-protein ligase activity Biological Process: cytokine and chemokine mediated signaling pathway; defense response to virus; innate immune response; ISG15-protein conjugation; negative regulation of interferon type I production; protein ubiquitination during ubiquitin-dependent protein catabolic process; regulation of cyclin-dependent protein kinase activity; regulation of defense response to virus |
NCBI Summary: | This gene is a member of the HERC family of ubiquitin ligases and encodes a protein with a HECT domain and five RCC1 repeats. Pro-inflammatory cytokines upregulate expression of this gene in endothelial cells. The protein localizes to the cytoplasm and perinuclear region and functions as an interferon-induced E3 protein ligase that mediates ISGylation of protein targets. The gene lies in a cluster of HERC family genes on chromosome 4. [provided by RefSeq, Jul 2008] |
UniProt Code: | Q9UII4 |
NCBI GenInfo Identifier: | 296434523 |
NCBI Gene ID: | 51191 |
NCBI Accession: | Q9UII4.2 |
UniProt Secondary Accession: | Q9UII4,Q69G20, B2RTQ1, |
UniProt Related Accession: | Q9UII4 |
Molecular Weight: | 116,852 Da |
NCBI Full Name: | E3 ISG15--protein ligase HERC5 |
NCBI Synonym Full Names: | HECT and RLD domain containing E3 ubiquitin protein ligase 5 |
NCBI Official Symbol: | HERC5 |
NCBI Official Synonym Symbols: | CEB1; CEBP1 |
NCBI Protein Information: | E3 ISG15--protein ligase HERC5 |
UniProt Protein Name: | E3 ISG15--protein ligase HERC5 |
UniProt Synonym Protein Names: | Cyclin-E-binding protein 1; HECT domain and RCC1-like domain-containing protein 5 |
Protein Family: | E3 ISG15--protein ligase |
UniProt Gene Name: | HERC5 |
UniProt Entry Name: | HERC5_HUMAN |
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Anti-HERC5 Antibody (CAB14889) |
Secondary Antibody |
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