GSPT1 Antibody (PACO55538)
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주문- SKU:
- PACO55538
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | GSPT1 Antibody (PACO55538) |
Antibody SKU: | PACO55538 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Eukaryotic peptide chain release factor GTP-binding subunit ERF3A protein (6-142AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
IHC image of PACO55538 diluted at 1:400 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
Immunofluorescence staining of Hela cells with PACO55538 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). | |
IHC image of PACO55538 diluted at 1:400 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated ABC system. |
Background: | Involved in translation termination in response to the termination codons UAA, UAG and UGA. Stimulates the activity of ERF1. Involved in regulation of mammalian cell growth. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. |
Synonyms: | Eukaryotic peptide chain release factor GTP-binding subunit ERF3A (Eukaryotic peptide chain release factor subunit 3a) (eRF3a) (G1 to S phase transition protein 1 homolog), GSPT1, ERF3A |
UniProt Protein Function: | Involved in translation termination in response to the termination codons UAA, UAG and UGA. Stimulates the activity of ERF1. Involved in regulation of mammalian cell growth. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. |
UniProt Code: | P15170 |
NCBI GenInfo Identifier: | 121688 |
NCBI Gene ID: | 2935 |
NCBI Accession: | P15170.1 |
UniProt Secondary Accession: | P15170,Q96GF2, J3KQG6, |
UniProt Related Accession: | P15170 |
Molecular Weight: | 68,700 Da |
NCBI Full Name: | Eukaryotic peptide chain release factor GTP-binding subunit ERF3A |
NCBI Synonym Full Names: | G1 to S phase transition 1 |
NCBI Official Symbol: | GSPT1 |
NCBI Official Synonym Symbols: | GST1; ETF3A; eRF3a; 551G9.2 |
NCBI Protein Information: | eukaryotic peptide chain release factor GTP-binding subunit ERF3A |
UniProt Protein Name: | Eukaryotic peptide chain release factor GTP-binding subunit ERF3A |
UniProt Synonym Protein Names: | G1 to S phase transition protein 1 homolog |
Protein Family: | Eukaryotic peptide chain release factor GTP-binding |
UniProt Gene Name: | GSPT1 |
UniProt Entry Name: | ERF3A_HUMAN |