GPT2 Antibody (PACO59245)
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주문- SKU:
- PACO59245
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | GPT2 Antibody (PACO59245) |
Antibody SKU: | PACO59245 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:500-1:1000 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Alanine aminotransferase 2 protein (95-208AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: U87 whole cell lysate. All lanes: GPT2 antibody at 4.5µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 58, 47 kDa. Observed band size: 58 kDa. | |
IHC image of PACO59245 diluted at 1:700 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
Background: | Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. |
Synonyms: | Alanine aminotransferase 2 (ALT2) (EC 2.6.1.2) (Glutamate pyruvate transaminase 2) (GPT 2) (Glutamic--alanine transaminase 2) (Glutamic--pyruvic transaminase 2), GPT2, AAT2 ALT2 |
UniProt Protein Function: | GPT2: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily. 2 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:EC 2.6.1.2; Amino Acid Metabolism - alanine, aspartate and glutamate; Transferase; Mitochondrial Chromosomal Location of Human Ortholog: 16q12.1 Cellular Component: mitochondrial matrix Molecular Function:alanine transaminase activity; pyridoxal phosphate binding Biological Process: 2-oxoglutarate metabolic process; amino acid biosynthetic process; L-alanine catabolic process; L-alanine metabolic process Disease: Mental Retardation, Autosomal Recessive 49 |
NCBI Summary: | This gene encodes a mitochondrial alanine transaminase, a pyridoxal enzyme that catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate. Alanine transaminases play roles in gluconeogenesis and amino acid metabolism in many tissues including skeletal muscle, kidney, and liver. Activating transcription factor 4 upregulates this gene under metabolic stress conditions in hepatocyte cell lines. A loss of function mutation in this gene has been associated with developmental encephalopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015] |
UniProt Code: | Q8TD30 |
NCBI GenInfo Identifier: | 74730602 |
NCBI Gene ID: | 84706 |
NCBI Accession: | Q8TD30.1 |
UniProt Secondary Accession: | Q8TD30,Q8N9E2, |
UniProt Related Accession: | Q8TD30 |
Molecular Weight: | 46,983 Da |
NCBI Full Name: | Alanine aminotransferase 2 |
NCBI Synonym Full Names: | glutamic pyruvate transaminase (alanine aminotransferase) 2 |
NCBI Official Symbol: | GPT2 |
NCBI Official Synonym Symbols: | ALT2; GPT 2; MRT49 |
NCBI Protein Information: | alanine aminotransferase 2 |
UniProt Protein Name: | Alanine aminotransferase 2 |
UniProt Synonym Protein Names: | Glutamate pyruvate transaminase 2; GPT 2; Glutamic--alanine transaminase 2; Glutamic--pyruvic transaminase 2 |
Protein Family: | Xanthine phosphoribosyltransferase |
UniProt Gene Name: | GPT2 |
UniProt Entry Name: | ALAT2_HUMAN |
Antibodies | ELISA Kits |
GPT2 Antibody (PACO05537) | Human ALT2 / Alanine aminotransferase 2 ELISA Kit |
GPT2 Antibody (PACO09576) | Human Alanine aminotransferase 2 (GPT2) ELISA Kit |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
Recommended Products |
Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |