GLIS3 Antibody (PACO61097)
제품 코드를 사용하여 Assay Genie 메인 사이트를 통해 주문합니다.
주문- SKU:
- PACO61097
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | GLIS3 Antibody (PACO61097) |
Antibody SKU: | PACO61097 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Zinc finger protein GLIS3 protein (527-663AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
IHC image of PACO61097 diluted at 1:300 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
Immunofluorescence staining of A549 cells with PACO61097 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
Background: | Acts as both a repressor and activator of transcription. Binds to the consensus sequence 5'-GACCACCCAC-3' (By similarity). |
Synonyms: | Zinc finger protein GLIS3 (GLI-similar 3) (Zinc finger protein 515), GLIS3, ZNF515 |
UniProt Protein Function: | GLIS3: Acts as both a repressor and activator of transcription. Binds to the consensus sequence 5'-GACCACCCAC-3'. Defects in GLIS3 are a cause of diabetes mellitus neonatal with congenital hypothyroidism (NDH). NDH is a neonatal diabetes syndrome associated with congenital hypothyroidism, congenital glaucoma, hepatic fibrosis and polycystic kidneys. Belongs to the GLI C2H2-type zinc-finger protein family. 2 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:C2H2-type zinc finger protein; Transcription factor Chromosomal Location of Human Ortholog: 9p24.2 Cellular Component: nucleus Biological Process: negative regulation of transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter Disease: Diabetes Mellitus, Neonatal, With Congenital Hypothyroidism |
NCBI Summary: | This gene is a member of the GLI-similar zinc finger protein family and encodes a nuclear protein with five C2H2-type zinc finger domains. This protein functions as both a repressor and activator of transcription and is specifically involved in the development of pancreatic beta cells, the thyroid, eye, liver and kidney. Mutations in this gene have been associated with neonatal diabetes and congenital hypothyroidism (NDH). Alternatively spliced variants that encode different protein isoforms have been described but the full-length nature of only two have been determined. [provided by RefSeq, Jul 2008] |
UniProt Code: | Q8NEA6 |
NCBI GenInfo Identifier: | 322510143 |
NCBI Gene ID: | 169792 |
NCBI Accession: | Q8NEA6.5 |
UniProt Secondary Accession: | Q8NEA6,Q1PHK5, B1AL19, |
UniProt Related Accession: | Q8NEA6 |
Molecular Weight: | 84kDa |
NCBI Full Name: | Zinc finger protein GLIS3 |
NCBI Synonym Full Names: | GLIS family zinc finger 3 |
NCBI Official Symbol: | GLIS3 |
NCBI Official Synonym Symbols: | NDH; ZNF515 |
NCBI Protein Information: | zinc finger protein GLIS3 |
UniProt Protein Name: | Zinc finger protein GLIS3 |
UniProt Synonym Protein Names: | GLI-similar 3; Zinc finger protein 515 |
Protein Family: | Zinc finger protein |
UniProt Gene Name: | GLIS3 |
Antibodies |
Anti-GLIS3 Antibody (CAB12166) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
Recommended Products |
Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |