GEMIN8 Antibody (PACO41194)
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주문- SKU:
- PACO41194
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | GEMIN8 Antibody (PACO41194) |
Antibody SKU: | PACO41194 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200 |
Species Reactivity: | Human, Mouse |
Immunogen: | Recombinant Human Gem-associated protein 8 protein (1-242AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Immunohistochemistry of paraffin-embedded human adrenal gland tissue using PACO41194 at dilution of 1:100. | |
Western Blot. Positive WB detected in: HepG2 whole cell lysate, HEK293 whole cell lysate, Mouse kidney tissue. All lanes: GEMIN8 antibody at 4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 29 kDa. Observed band size: 29, 33 kDa. |
Background: | The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus. |
Synonyms: | Gem-associated protein 8 (Gemin-8) (Protein FAM51A1), GEMIN8, FAM51A1 |
UniProt Protein Function: | GEMIN8: The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. |
UniProt Protein Details: | Protein type:RNA splicing; RNA-binding Chromosomal Location of Human Ortholog: Xp22.2 Cellular Component: cytoplasm; cytosol; nucleus; SMN complex Molecular Function:protein binding Biological Process: spliceosomal snRNP biogenesis |
NCBI Summary: | The protein encoded by this gene is part of the SMN complex, which is necessary for spliceosomal snRNP assembly in the cytoplasm and pre-mRNA splicing in the nucleus. The encoded protein binds to both SMN1 and the GEMIN6/GEMIN7 heterodimer, mediating their interaction. This protein is found in nuclear Gemini of Cajal bodies (gems) and in the cytoplasm. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, May 2010] |
UniProt Code: | Q9NWZ8 |
NCBI GenInfo Identifier: | 52782795 |
NCBI Gene ID: | 54960 |
NCBI Accession: | Q9NWZ8.1 |
UniProt Secondary Accession: | Q9NWZ8,Q2LJ66, Q6ZV27, C4AMC4, |
UniProt Related Accession: | Q9NWZ8 |
Molecular Weight: | 28,637 Da |
NCBI Full Name: | Gem-associated protein 8 |
NCBI Synonym Full Names: | gem nuclear organelle associated protein 8 |
NCBI Official Symbol: | GEMIN8 |
NCBI Official Synonym Symbols: | FAM51A1 |
NCBI Protein Information: | gem-associated protein 8 |
UniProt Protein Name: | Gem-associated protein 8 |
UniProt Synonym Protein Names: | Protein FAM51A1 |
Protein Family: | Gem-associated protein |
UniProt Gene Name: | GEMIN8 |
UniProt Entry Name: | GEMI8_HUMAN |