GEMIN4 Antibody (PACO57776)
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주문- SKU:
- PACO57776
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | GEMIN4 Antibody (PACO57776) |
Antibody SKU: | PACO57776 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Gem-associated protein 4 protein (741-848AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
IHC image of PACO57776 diluted at 1:400 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
Immunofluorescence staining of A549 cells with PACO57776 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
Background: | The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus. |
Synonyms: | Gem-associated protein 4 (Gemin-4) (Component of gems 4) (p97), GEMIN4 |
UniProt Protein Function: | GEMIN4: The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. |
UniProt Protein Details: | Protein type:Nucleolus; RNA splicing; RNA-binding Chromosomal Location of Human Ortholog: 17p13.3 Cellular Component: cytoplasm; cytosol; membrane; nuclear body; nucleoplasm; small nuclear ribonucleoprotein complex; SMN complex Molecular Function:protein binding Biological Process: nuclear import; spliceosomal snRNP biogenesis |
NCBI Summary: | The product of this gene is part of a large complex localized to the cytoplasm, nucleoli, and to discrete nuclear bodies called Gemini bodies (gems). The complex functions in spliceosomal snRNP assembly in the cytoplasm, and regenerates spliceosomes required for pre-mRNA splicing in the nucleus. The encoded protein directly interacts with a DEAD box protein and several spliceosome core proteins. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008] |
UniProt Code: | P57678 |
NCBI GenInfo Identifier: | 322510030 |
NCBI Gene ID: | 50628 |
NCBI Accession: | P57678.2 |
UniProt Secondary Accession: | P57678,Q9NZS7, Q9UG32, Q9Y4Q2, |
UniProt Related Accession: | P57678 |
Molecular Weight: | 120kDa |
NCBI Full Name: | Gem-associated protein 4 |
NCBI Synonym Full Names: | gem nuclear organelle associated protein 4 |
NCBI Official Symbol: | GEMIN4 |
NCBI Official Synonym Symbols: | p97; HC56; HCAP1; HHRF-1 |
NCBI Protein Information: | gem-associated protein 4 |
UniProt Protein Name: | Gem-associated protein 4 |
UniProt Synonym Protein Names: | Component of gems 4; p97 |
Protein Family: | Gem-associated protein |
UniProt Gene Name: | GEMIN4 |