GABARAPL2 Antibody (PACO31692)
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주문- SKU:
- PACO31692
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | GABARAPL2 Antibody (PACO31692) |
Antibody SKU: | PACO31692 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:1000-1:2000 |
Species Reactivity: | Human, Rat |
Immunogen: | Recombinant Human Gamma-aminobutyric acid, receptor-associated protein-like 2 protein (1-117AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western Blot Positive WB detected in: Raji whole cell lysate, Rat spleen tissue All lanes: GABARAPL2 antibody at 4µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 14 kDa Observed band size: 14 kDa . | |
IHC image of PACO31692 diluted at 1:1200 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
IHC image of PACO31692 diluted at 1:1200 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
Background: | Ubiquitin-like modifier involved in intra-Golgi traffic. Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1 (By similarity). Involved in autophagy. Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. |
Synonyms: | Gamma-aminobutyric acid, receptor-associated protein-like 2 (GABA(A) receptor-associated protein-like 2) (Ganglioside expression factor 2) (GEF-2) (General protein transport factor p16) (Golgi-associated ATPase enhancer of 16 kDa) (GATE-16) (MAP1 light chain 3-related protein), GABARAPL2, FLC3A GEF2 |
UniProt Protein Function: | GABARAPL2: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. Implicated in intra-Golgi transport and post-mitotic Golgi re-assembly. Modulates the activity of SNAREs in the Golgi apparatus and is therefore an essential component of intra-Golgi transport and post-mitotic Golgi re-assembly. It first stimulates the ATPase activity of NSF, which in turn stimulates the association with GOSR1. Interacts with GABRG2, NSF, GOSR1 and beta-tubulin. Interacts with ULK1. Phosphorylated upon DNA damage, probably by ATM or ATR. |
UniProt Protein Details: | Protein type:Vesicle; Microtubule-binding; Ubiquitin-like modifier; Autophagy; Adaptor/scaffold Chromosomal Location of Human Ortholog: 16q22.1 Cellular Component: autophagic vacuole; cytoplasm; cytosol; Golgi apparatus; Golgi membrane; intracellular Molecular Function:ATPase binding; beta-tubulin binding; GABA receptor binding; protein binding; SNARE binding; ubiquitin protein ligase binding Biological Process: autophagic vacuole formation; cellular response to nitrogen starvation; intra-Golgi vesicle-mediated transport; macroautophagy; mitochondrion degradation; positive regulation of ATPase activity |
UniProt Code: | P60520 |
NCBI GenInfo Identifier: | 44888808 |
NCBI Gene ID: | 11345 |
NCBI Accession: | P60520.1 |
UniProt Secondary Accession: | P60520,O08765, Q6FG91, Q9DCP8, Q9UQF7, |
UniProt Related Accession: | P60520 |
Molecular Weight: | 13,667 Da |
NCBI Full Name: | Gamma-aminobutyric acid receptor-associated protein-like 2 |
NCBI Synonym Full Names: | GABA type A receptor associated protein like 2 |
NCBI Official Symbol: | GABARAPL2 |
NCBI Official Synonym Symbols: | ATG8; GEF2; ATG8C; GEF-2; GATE16; GATE-16 |
NCBI Protein Information: | gamma-aminobutyric acid receptor-associated protein-like 2 |
UniProt Protein Name: | Gamma-aminobutyric acid receptor-associated protein-like 2 |
UniProt Synonym Protein Names: | GABA(A) receptor-associated protein-like 2; Ganglioside expression factor 2; GEF-2; General protein transport factor p16; Golgi-associated ATPase enhancer of 16 kDa; GATE-16; MAP1 light chain 3-related protein |
Protein Family: | Gamma-aminobutyric acid receptor-associated protein |
UniProt Gene Name: | GABARAPL2 |
UniProt Entry Name: | GBRL2_HUMAN |
Antibodies |
Anti-GABARAPL2 Antibody (CAB14855) |
Anti-GABARAPL2 Antibody (CAB7782) |
Anti-GABARAPL2 Antibody (CAB9585) |
GABARAPL2 Antibody (PACO09379) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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