FZD7 Antibody (PACO62903)
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주문- SKU:
- PACO62903
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
Antibody Name: | FZD7 Antibody (PACO62903) |
Antibody SKU: | PACO62903 |
Size: | 50ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:200-1:500 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Frizzled-7 protein (161-239AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: HepG2 whole cell lysate, 293T whole cell lysate. All lanes: FZD7 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 64 kDa. Observed band size: 64 kDa. | |
IHC image of PACO62903 diluted at 1:400 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
Background: | Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. |
Synonyms: | Frizzled-7 (Fz-7) (hFz7) (FzE3), FZD7 |
UniProt Protein Function: | FZD7: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK- 3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Belongs to the G-protein coupled receptor Fz/Smo family. |
UniProt Protein Details: | Protein type:Receptor, GPCR; Motility/polarity/chemotaxis; Membrane protein, multi-pass; GPCR, Fz/Smo family; Membrane protein, integral Chromosomal Location of Human Ortholog: 2q33 Cellular Component: integral to membrane; plasma membrane Molecular Function:G-protein coupled receptor activity; Wnt-protein binding; Wnt receptor activity; protein binding; frizzled binding; PDZ domain binding Biological Process: neuron differentiation; G-protein coupled receptor protein signaling pathway; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; positive regulation of epithelial cell proliferation involved in wound healing; stem cell maintenance; T cell differentiation in the thymus; positive regulation of JNK cascade; somatic stem cell division; Wnt receptor signaling pathway through beta-catenin; satellite cell compartment self-renewal involved in skeletal muscle regeneration; negative regulation of ectodermal cell fate specification; positive regulation of phosphorylation |
NCBI Summary: | Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD7 protein contains an N-terminal signal sequence, 10 cysteine residues typical of the cysteine-rich extracellular domain of Fz family members, 7 putative transmembrane domains, and an intracellular C-terminal tail with a PDZ domain-binding motif. FZD7 gene expression may downregulate APC function and enhance beta-catenin-mediated signals in poorly differentiated human esophageal carcinomas. [provided by RefSeq, Jul 2008] |
UniProt Code: | O75084 |
NCBI GenInfo Identifier: | 52788226 |
NCBI Gene ID: | 8324 |
NCBI Accession: | O75084.2 |
UniProt Related Accession: | O75084 |
Molecular Weight: | ~ 64, 85kDa |
NCBI Full Name: | Frizzled-7 |
NCBI Synonym Full Names: | frizzled class receptor 7 |
NCBI Official Symbol: | FZD7 |
NCBI Official Synonym Symbols: | FzE3 |
NCBI Protein Information: | frizzled-7 |
UniProt Protein Name: | Frizzled-7 |
UniProt Synonym Protein Names: | FzE3 |
Protein Family: | Frizzled |
UniProt Gene Name: | FZD7 |
UniProt Entry Name: | FZD7_HUMAN |