EIF3A Antibody (PACO52398)
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주문- SKU:
- PACO52398
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | EIF3A Antibody (PACO52398) |
Antibody SKU: | PACO52398 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IF:1:50-1:200 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Eukaryotic translation initiation factor 3 subunit A protein (1288-1379AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: Jurkat whole cell lysate. All lanes: EIF3A antibody at 4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 167, 163 kDa. Observed band size: 167 kDa. | |
Immunofluorescent analysis of PC-3 cells using PACO52398 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
Background: | Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Essential for the initiation of translation on type-1 viral ribosomal entry sites (IRESs), like for HCV, PV, EV71 or BEV translation. In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2. |
Synonyms: | Eukaryotic translation initiation factor 3 subunit A (eIF3a) (Eukaryotic translation initiation factor 3 subunit 10) (eIF-3-theta) (eIF3 p167) (eIF3 p180) (eIF3 p185), EIF3A, EIF3S10 KIAA0139 |
UniProt Protein Function: | eIF3-theta: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Interacts with EIF4G1. Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3L and EIF3K. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Also interacts with KRT7 and PIWIL2. Belongs to the eIF-3 subunit A family. |
UniProt Protein Details: | Protein type:Translation; RNA-binding; Translation initiation Chromosomal Location of Human Ortholog: 10q26 Cellular Component: cytoplasm; cytosol; eukaryotic translation initiation factor 3 complex; membrane; nucleolus; nucleus Molecular Function:protein binding; translation initiation factor activity Biological Process: formation of translation initiation complex; translational initiation |
UniProt Code: | Q14152 |
NCBI GenInfo Identifier: | 6685537 |
NCBI Gene ID: | 8661 |
NCBI Accession: | Q14152.1 |
UniProt Secondary Accession: | Q14152,O00653, Q15778, B1AMV5, B4DYS1, F5H335, |
UniProt Related Accession: | Q14152 |
Molecular Weight: | 162,636 Da |
NCBI Full Name: | Eukaryotic translation initiation factor 3 subunit A |
NCBI Synonym Full Names: | eukaryotic translation initiation factor 3 subunit A |
NCBI Official Symbol: | EIF3A |
NCBI Official Synonym Symbols: | EIF3; P167; p180; p185; TIF32; EIF3S10; eIF3-p170; eIF3-theta |
NCBI Protein Information: | eukaryotic translation initiation factor 3 subunit A |
UniProt Protein Name: | Eukaryotic translation initiation factor 3 subunit A |
UniProt Synonym Protein Names: | Eukaryotic translation initiation factor 3 subunit 10 |
Protein Family: | Eukaryotic translation initiation factor |
UniProt Gene Name: | EIF3A |
UniProt Entry Name: | EIF3A_HUMAN |
Antibodies | ELISA Kits |
Anti-EIF3A Antibody (CAB0573) | Human eIF3A ELISA Kit |
Human Eukaryotic translation initiation factor 3 subunit A (EIF3A) ELISA Kit |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
Recommended Products |
Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |