EIF2S3 Antibody (PACO44332)
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주문- SKU:
- PACO44332
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | EIF2S3 Antibody (PACO44332) |
Antibody SKU: | PACO44332 |
Size: | 50ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:20-1:200 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Eukaryotic translation initiation factor 2 subunit 3 protein (173-472AA) |
Form: | Liquid |
Storage Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Purification Method: | Antigen Affinity Purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western blot. All lanes: EIF2S3 antibody at 1.13µg/ml + Hela whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 51 kDa. Observed band size: 51 kDa.. | |
Immunohistochemistry of paraffin-embedded human tonsil tissue using PACO44332 at dilution of 1:100. |
Background: | As a subunit of eukaryotic initiation factor 2 (eIF2), involved in the early steps of protein synthesis. In the presence of GTP, eIF2 forms a ternary complex with initiator tRNA Met-tRNAi and then recruits the 40S ribosomal complex, a step that determines the rate of protein translation. This step is followed by mRNA binding to form the 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF2 and release of an eIF2-GDP binary complex. In order for eIF2 to recycle and catalyze another round of initiation, the GDP bound to eIF2 must exchange with GTP by way of a reaction catalyzed by eIF2B (By similarity). Along with its paralog on chromosome Y, may contribute to spermatogenesis up to the round spermatid stage (By similarity). |
Synonyms: | Eukaryotic translation initiation factor 2 subunit 3 (Eukaryotic translation initiation factor 2 subunit γ X) (eIF-2-γ X) (eIF-2gX), EIF2S3, EIF2G |
UniProt Protein Function: | eIF2-gamma: eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. |
UniProt Protein Details: | Protein type:Translation initiation; Translation Chromosomal Location of Human Ortholog: Xp22.2-p22.1 Cellular Component: cytoplasm; cytosol; nucleus Molecular Function:protein binding; translation factor activity, nucleic acid binding; translation initiation factor activity Biological Process: formation of translation preinitiation complex; translational initiation; transmembrane transport |
NCBI Summary: | The protein encoded by this gene is the largest subunit of a heterotrimeric GTP-binding protein involved in the recruitment of methionyl-tRNA(i) to the 40 S ribosomal subunit. [provided by RefSeq, Jan 2010] |
UniProt Code: | P41091 |
NCBI GenInfo Identifier: | 729816 |
NCBI Gene ID: | 1968 |
NCBI Accession: | P41091.3 |
UniProt Secondary Accession: | P41091,Q53HK3, A0A024RBY4, A8K2Y2, B2R5N2, B5BTZ4, |
UniProt Related Accession: | P41091 |
Molecular Weight: | 51,109 Da |
NCBI Full Name: | Eukaryotic translation initiation factor 2 subunit 3 |
NCBI Synonym Full Names: | eukaryotic translation initiation factor 2 subunit gamma |
NCBI Official Symbol: | EIF2S3 |
NCBI Official Synonym Symbols: | EIF2; EIF2G; eIF-2gA; EIF2gamma |
NCBI Protein Information: | eukaryotic translation initiation factor 2 subunit 3 |
UniProt Protein Name: | Eukaryotic translation initiation factor 2 subunit 3 |
UniProt Synonym Protein Names: | Eukaryotic translation initiation factor 2 subunit gamma X; eIF-2-gamma X; eIF-2gX |
Protein Family: | Eukaryotic translation initiation factor |
UniProt Gene Name: | EIF2S3 |
UniProt Entry Name: | IF2G_HUMAN |