DNA Polymerase Beta/POLB Recombinant Protein (RPES2462)

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SKU:
RPES2462
Product type:
Recombinant Protein
Host species:
E.coli
Reactivity:
Human
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Description

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DNA Polymerase Beta/POLB Recombinant Protein

Human DNA polymerase β is constitutively expressed in cells. It fills in gaps in DNA that are formed following base excision repair. Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. It conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. The activity cannot be affected by Aphidicolin, which is an inhibitor of DNA polymerase β.

Product Name:DNA Polymerase Beta/POLB Recombinant Protein (RPES2462)
Product Code:RPES2462
Size:10µg
Species:Human
Expressed Host:E.coli
Synonyms:DNA Polymerase Beta, POLB
Accession:P06746
Sequence:Ser2-Glu335
Fusion tag:C-6His
Endotoxin:<1.0 EU per µg as determined by the LAL method.
Protein Construction:Recombinant Human DNA Polymerase beta is produced by our E.coli expression system and the target gene encoding Ser2-Glu335 is expressed with a 6His tag at the C-terminus.
Purity:> 95 % as determined by reducing SDS-PAGE.
Mol Mass:39.2 kDa
AP Mol Mass:39 kDa
Formulation:Supplied as a 0.2 µm filtered solution of 20mM TrisHCl, 100mM NaCl, 1mM DTT, 1mM EDTA, 50% Glycerol, pH 7.8.
Shipping:This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
Stability and Storage:Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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