DCP2 Antibody (PACO43725)
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주문- SKU:
- PACO43725
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | DCP2 Antibody (PACO43725) |
Antibody SKU: | PACO43725 |
Size: | 50ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:200-1:1000, IHC:1:20-1:200 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human m7GpppN-mRNA hydrolase protein (1-240AA) |
Form: | Liquid |
Storage Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Purification Method: | Antigen Affinity Purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western blot. All lanes: m7GpppN-mRNA hydrolase antibody at 7µg/ml. Lane 1: Hela whole cell lysate. Lane 2: Jurkat whole cell lysate. Lane 3: 293T whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 49, 45 kDa. Observed band size: 49 kDa. | |
Immunohistochemistry of paraffin-embedded human kidney tissue using PACO43725 at dilution of 1:100. | |
Immunohistochemistry of paraffin-embedded human small intestine tissue using PACO43725 at dilution of 1:100. |
Background: | Decapping metalloenzyme that catalyzes the cleavage of the cap structure on mRNAs. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Plays a role in replication-dependent histone mRNA degradation. Has higher activity towards mRNAs that lack a poly(A) tail. Has no activity towards a cap structure lacking an RNA moiety. |
Synonyms: | m7GpppN-mRNA hydrolase (EC 3.6.1.62) (Nucleoside diphosphate-linked moiety X motif 20) (Nudix motif 20) (mRNA-decapping enzyme 2) (hDpc), DCP2, NUDT20 |
UniProt Protein Function: | DCP2: Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Plays a role in replication-dependent histone mRNA degradation. Removes the 7- methyl guanine cap structure from mRNA molecules, yielding a 5'- phosphorylated mRNA fragment and 7m-GDP. Has higher activity towards mRNAs that lack a poly(A) tail. Has no activity towards a cap structure lacking a RNA moiety. Belongs to the Nudix hydrolase family. DCP2 subfamily. 2 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:EC 3.6.1.62; Hydrolase; RNA processing Chromosomal Location of Human Ortholog: 5q22.2 Cellular Component: cell junction; cytosol; intracellular membrane-bound organelle; nucleoplasm Molecular Function:exoribonuclease activity, producing 5'-phosphomonoesters; m7G(5')pppN diphosphatase activity; protein binding Biological Process: deadenylation-dependent decapping; mRNA catabolic process; mRNA catabolic process, nonsense-mediated decay; regulation of mRNA stability |
NCBI Summary: | The protein encoded by this gene is a key component of an mRNA-decapping complex required for degradation of mRNAs, both in normal mRNA turnover, and in nonsense-mediated mRNA decay (NMD). It removes the 7-methyl guanine cap structure from mRNA, prior to its degradation from the 5' end. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Jun 2011] |
UniProt Code: | Q8IU60 |
NCBI GenInfo Identifier: | 269849560 |
NCBI Gene ID: | 167227 |
NCBI Accession: | Q8IU60.2 |
UniProt Secondary Accession: | Q8IU60,Q6P2D4, Q7Z5W5, Q8NBG5, C9J778, |
UniProt Related Accession: | Q8IU60 |
Molecular Weight: | 44,443 Da |
NCBI Full Name: | m7GpppN-mRNA hydrolase |
NCBI Synonym Full Names: | decapping mRNA 2 |
NCBI Official Symbol: | DCP2 |
NCBI Official Synonym Symbols: | NUDT20 |
NCBI Protein Information: | m7GpppN-mRNA hydrolase |
UniProt Protein Name: | m7GpppN-mRNA hydrolase |
UniProt Synonym Protein Names: | Nucleoside diphosphate-linked moiety X motif 20; Nudix motif 20; mRNA-decapping enzyme 2; hDpc |
Protein Family: | mRNA-decapping enzyme |
UniProt Gene Name: | DCP2 |
UniProt Entry Name: | DCP2_HUMAN |
Antibodies |
Anti-DCP2 Antibody (CAB8282) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
Recommended Products |
Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |