DAG1 Antibody (PACO63067)
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주문- SKU:
- PACO63067
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
Antibody Name: | DAG1 Antibody (PACO63067) |
Antibody SKU: | PACO63067 |
Size: | 50ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, IHC |
Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:200-1:500 |
Species Reactivity: | Human |
Immunogen: | Peptide sequence from Human Dystroglycan protein (726-744AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
IHC image of PACO63067 diluted at 1:300 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
Background: | The dystroglycan complex is involved in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization. |
Synonyms: | Dystroglycan (Dystrophin-associated glycoprotein 1) [Cleaved into: Alpha-dystroglycan (Alpha-DG); Beta-dystroglycan (Beta-DG)], DAG1 |
UniProt Protein Function: | DAG1: a cytoskeletal protein that functions as a laminin receptor. Provides a linkage between the subsarcolemmal cytoskeleton and the extracellular matrix. May be involved in autosomal recessive muscular dystrophies. Its dramatic reduction in Duchenne muscular dystrophy leads to a loss of linkage between the sarcolemma and extracellular matrix, rendering muscle fibers more susceptible to necrosis. Dystroglycan also functions as dual receptor for agrin and laminin-2 in the Schwann cell membrane. Binds to several types of arenaviruses. Is a target for the entry of Mycobacterium leprae into peripheral nerve Schwann cells. The muscle and nonmuscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence. |
UniProt Protein Details: | Protein type:Cytoskeletal; Motility/polarity/chemotaxis; Membrane protein, integral Chromosomal Location of Human Ortholog: 3p21 Cellular Component: dystroglycan complex; extracellular space; costamere; focal adhesion; basolateral plasma membrane; integral to membrane; extracellular region; lipid raft; nucleoplasm; dystrophin-associated glycoprotein complex; postsynaptic membrane; cytoskeleton; cell-cell adherens junction; lamellipodium; cytoplasm; plasma membrane; basement membrane; sarcolemma; filopodium Molecular Function:tubulin binding; viral receptor activity; laminin-1 binding; protein binding; structural constituent of muscle; protein complex binding; SH2 domain binding; calcium ion binding; alpha-actinin binding; actin binding; vinculin binding Biological Process: nerve maturation; response to peptide hormone stimulus; entry of virus into host cell; extracellular matrix organization and biogenesis; negative regulation of protein kinase B signaling cascade; cytoskeletal anchoring; negative regulation of MAPKKK cascade; membrane protein ectodomain proteolysis; NLS-bearing substrate import into nucleus; myelination in the peripheral nervous system; virus-host interaction; morphogenesis of an epithelial sheet; calcium-dependent cell-matrix adhesion; negative regulation of cell migration Disease: Muscular Dystrophy-dystroglycanopathy (limb-girdle), Type C, 9; Muscular Dystrophy-dystroglycanopathy (congenital With Brain And Eye Anomalies), Type A, 9 |
NCBI Summary: | Dystroglycan is a laminin binding component of the dystrophin-glycoprotein complex which provides a linkage between the subsarcolemmal cytoskeleton and the extracellular matrix. Dystroglycan 1 is a candidate gene for the site of the mutation in autosomal recessive muscular dystrophies. The dramatic reduction of dystroglycan 1 in Duchenne muscular dystrophy leads to a loss of linkage between the sarcolemma and extracellular matrix, rendering muscle fibers more susceptible to necrosis. Dystroglycan also functions as dual receptor for agrin and laminin-2 in the Schwann cell membrane. The muscle and nonmuscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence. Alternative splicing results in multiple transcript variants all encoding the same protein.[provided by RefSeq, Apr 2010] |
UniProt Code: | Q14118 |
NCBI GenInfo Identifier: | 229462879 |
NCBI Gene ID: | 1605 |
NCBI Accession: | Q14118.2 |
UniProt Secondary Accession: | Q14118,Q969J9, A8K6M7, |
UniProt Related Accession: | Q14118 |
Molecular Weight: | 97,441 Da |
NCBI Full Name: | Dystroglycan |
NCBI Synonym Full Names: | dystroglycan 1 (dystrophin-associated glycoprotein 1) |
NCBI Official Symbol: | DAG1 |
NCBI Official Synonym Symbols: | A3a; DAG; AGRNR; 156DAG; MDDGC7; MDDGC9 |
NCBI Protein Information: | dystroglycan |
UniProt Protein Name: | Dystroglycan |
UniProt Synonym Protein Names: | Dystrophin-associated glycoprotein 1 |
Protein Family: | Dystroglycan |
UniProt Gene Name: | DAG1 |
UniProt Entry Name: | DAG1_HUMAN |
Antibodies | ELISA Kits |
Anti-DAG1 Antibody (CAB10076) | Human DAG1 / Dystroglycan ELISA Kit |
DAG1 Antibody (PACO08736) | Human DAG1 (Dystrophin Associated Glycoprotein 1) CLIA Kit (HUES01283) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
Recommended Products |
Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |