CXCR3 Antibody (PACO45758)
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주문- SKU:
- PACO45758
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
Antibody Name: | CXCR3 Antibody (PACO45758) |
Antibody SKU: | PACO45758 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:1000-1:2000, IF:1:50-1:500 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human C-X-C chemokine receptor type 3 protein (4-50AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: K562 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate. All lanes: CXCR3 antibody at 3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 41, 46, 29 kDa. Observed band size: 41 kDa.. | |
IHC image of PACO45758 diluted at 1:1200 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
IHC image of PACO45758 diluted at 1:1200 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
Background: | Isoform 1: Receptor for the C-X-C chemokine CXCL9, CXCL10 and CXCL11 and mediates the proliferation, survival and angiogenic activity of human mesangial cells (HMC) through a heterotrimeric G-protein signaling pathway. Binds to CCL21. Probably promotes cell chemotaxis response. |
Synonyms: | C-X-C chemokine receptor type 3 (CXC-R3) (CXCR-3) (CKR-L2) (G protein-coupled receptor 9) (Interferon-inducible protein 10 receptor) (IP-10 receptor) (CD antigen CD183), CXCR3, GPR9 |
UniProt Protein Function: | CXCR3: Receptor for CXCL9, CXCL10 and CXCL11 and mediates the proliferation of human mesangial cells (HMC). Belongs to the G-protein coupled receptor 1 family. 3 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Membrane protein, integral; GPCR, family 1; Receptor, GPCR; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: Xq13 Cellular Component: integral to plasma membrane; cytoplasm; plasma membrane; external side of plasma membrane Molecular Function:C-X-C chemokine binding; chemokine binding; chemokine receptor activity; receptor activity; C-X-C chemokine receptor activity Biological Process: positive regulation of chemotaxis; apoptosis; calcium-mediated signaling; positive regulation of cAMP metabolic process; chemotaxis; G-protein coupled receptor protein signaling pathway; positive regulation of angiogenesis; negative regulation of angiogenesis; elevation of cytosolic calcium ion concentration; cell surface receptor linked signal transduction; negative regulation of endothelial cell proliferation; positive regulation of cell proliferation; regulation of leukocyte migration; positive regulation of transcription from RNA polymerase II promoter; angiogenesis; positive regulation of release of sequestered calcium ion into cytosol; inflammatory response; cell motility; cell adhesion |
NCBI Summary: | This gene encodes a G protein-coupled receptor with selectivity for three chemokines, termed CXCL9/Mig (monokine induced by interferon-g), CXCL10/IP10 (interferon-g-inducible 10 kDa protein) and CXCL11/I-TAC (interferon-inducible T cell a-chemoattractant). Binding of chemokines to this protein induces cellular responses that are involved in leukocyte traffic, most notably integrin activation, cytoskeletal changes and chemotactic migration. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. One of the isoforms (CXCR3-B) shows high affinity binding to chemokine, CXCL4/PF4 (PMID:12782716). [provided by RefSeq, Jun 2011] |
UniProt Code: | P49682 |
NCBI GenInfo Identifier: | 2829400 |
NCBI Gene ID: | 2833 |
NCBI Accession: | P49682.2 |
UniProt Secondary Accession: | P49682,O15185, Q7Z710, Q9P2T4, Q9P2T5, B2R982, |
UniProt Related Accession: | P49682 |
Molecular Weight: | Observed MW: 44kDaCalculated MW: 28kDa/40kDa/45kDa |
NCBI Full Name: | C-X-C chemokine receptor type 3 |
NCBI Synonym Full Names: | chemokine (C-X-C motif) receptor 3 |
NCBI Official Symbol: | CXCR3 |
NCBI Official Synonym Symbols: | GPR9; MigR; CD182; CD183; Mig-R; CKR-L2; CMKAR3; IP10-R |
NCBI Protein Information: | C-X-C chemokine receptor type 3; CXC-R3; CXCR-3; Mig receptor; IP10 receptor; IP-10 receptor; G protein-coupled receptor 9; chemokine (C-X-C) receptor 3; interferon-inducible protein 10 receptor |
UniProt Protein Name: | C-X-C chemokine receptor type 3 |
UniProt Synonym Protein Names: | CKR-L2; G protein-coupled receptor 9; Interferon-inducible protein 10 receptor; IP-10 receptor; CD_antigen: CD183 |
Protein Family: | C-X-C chemokine receptor |
UniProt Gene Name: | CXCR3 |
UniProt Entry Name: | CXCR3_HUMAN |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
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