CHRNB2 Antibody (PACO56752)
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주문- SKU:
- PACO56752
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
Antibody Name: | CHRNB2 Antibody (PACO56752) |
Antibody SKU: | PACO56752 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200 |
Species Reactivity: | Human, Rat |
Immunogen: | Recombinant Human Neuronal acetylcholine receptor subunit beta-2 protein (322-458AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western Blot. Positive WB detected in: Jurkat whole cell lysate. All lanes: CHRNB2 antibody at 6.05µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 58 kDa. Observed band size: 58 kDa. | |
IHC image of PACO56752 diluted at 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
Immunofluorescence staining of SH-SY5Y cells with PACO56752 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
Background: | After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane permeable to sodiun ions. |
Synonyms: | Neuronal acetylcholine receptor subunit beta-2, CHRNB2 |
UniProt Protein Function: | nAChRB2: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. Defects in CHRNB2 are the cause of nocturnal frontal lobe epilepsy type 3 (ENFL3). ENFL3 is an autosomal dominant epilepsy characterized by nocturnal seizures with hyperkinetic automatisms and poorly organized stereotyped movements. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Beta- 2/CHRNB2 sub-subfamily. |
UniProt Protein Details: | Protein type:Receptor, misc.; Channel, cation; Membrane protein, multi-pass; Channel, ligand-gated; Membrane protein, integral Chromosomal Location of Human Ortholog: 1q21.3 Cellular Component: external side of plasma membrane; nicotinic acetylcholine-gated receptor-channel complex; plasma membrane Molecular Function:acetylcholine binding; acetylcholine receptor activity; ligand-gated ion channel activity; nicotinic acetylcholine-activated cation-selective channel activity; protein binding Biological Process: associative learning; B cell activation; behavioral response to nicotine; calcium ion transport; central nervous system projection neuron axonogenesis; cognition; lateral geniculate nucleus development; learning; locomotory behavior; membrane depolarization; memory; negative regulation of action potential; neurological system process; neuromuscular synaptic transmission; optic nerve morphogenesis; positive regulation of B cell proliferation; positive regulation of dopamine secretion; regulation of circadian sleep/wake cycle, REM sleep; regulation of dendrite morphogenesis; regulation of dopamine metabolic process; regulation of dopamine secretion; regulation of synaptic transmission, dopaminergic; regulation of synaptogenesis; response to cocaine; response to ethanol; response to hypoxia; response to nicotine; sensory perception of pain; sensory perception of sound; signal transduction; smooth muscle contraction; social behavior; synaptic transmission involved in micturition; synaptic transmission, cholinergic; vestibulocochlear nerve development; visual learning; visual perception Disease: Epilepsy, Nocturnal Frontal Lobe, 3 |
NCBI Summary: | Neuronal acetylcholine receptors are homo- or heteropentameric complexes composed of homologous alpha and beta subunits. They belong to a superfamily of ligand-gated ion channels which allow the flow of sodium and potassium across the plasma membrane in response to ligands such as acetylcholine and nicotine. This gene encodes one of several beta subunits. Mutations in this gene are associated with autosomal dominant nocturnal frontal lobe epilepsy. [provided by RefSeq, Jul 2008] |
UniProt Code: | P17787 |
NCBI GenInfo Identifier: | 113105 |
NCBI Gene ID: | 1141 |
NCBI Accession: | P17787.1 |
UniProt Secondary Accession: | P17787,Q9UEH9, |
UniProt Related Accession: | P17787 |
Molecular Weight: | 57,019 Da |
NCBI Full Name: | Neuronal acetylcholine receptor subunit beta-2 |
NCBI Synonym Full Names: | cholinergic receptor nicotinic beta 2 subunit |
NCBI Official Symbol: | CHRNB2 |
NCBI Official Synonym Symbols: | EFNL3; nAChRB2 |
NCBI Protein Information: | neuronal acetylcholine receptor subunit beta-2 |
UniProt Protein Name: | Neuronal acetylcholine receptor subunit beta-2 |
Protein Family: | Neuronal acetylcholine receptor |
UniProt Gene Name: | CHRNB2 |
UniProt Entry Name: | ACHB2_HUMAN |