CD99L2 Antibody (PACO57348)
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주문- SKU:
- PACO57348
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | CD99L2 Antibody (PACO57348) |
Antibody SKU: | PACO57348 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human CD99 antigen-like protein 2 protein (26-145AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
IHC image of PACO57348 diluted at 1:400 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. | |
Western Blot. Positive WB detected in: SH-SY5Y whole cell lysate. All lanes: CD99L2 antibody at 4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 28, 23, 21, 16, 29 kDa. Observed band size: 28 kDa. | |
Immunofluorescence staining of A549 cells with PACO57348 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
Background: | Plays a role in a late step of leukocyte extravasation helping cells to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1 (By similarity). Homophilic adhesion molecule, but these interactions may not be required for cell aggregation (By similarity). |
Synonyms: | CD99 antigen-like protein 2 (MIC2-like protein 1) (CD antigen CD99), CD99L2, MIC2L1 |
UniProt Protein Function: | CD99L2: Plays a role in a late step of leukocyte extravasation helping cells to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Homophilic adhesion molecule, but these interactions may not be required for cell aggregation. Belongs to the CD99 family. 5 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Cell adhesion; Membrane protein, integral Chromosomal Location of Human Ortholog: Xq28 Cellular Component: focal adhesion; integral to membrane; plasma membrane Biological Process: cell adhesion |
NCBI Summary: | This gene encodes a cell-surface protein that is similar to CD99. A similar protein in mouse functions as an adhesion molecule during leukocyte extravasation. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2010] |
UniProt Code: | Q8TCZ2 |
NCBI GenInfo Identifier: | 296317276 |
NCBI Gene ID: | 83692 |
NCBI Accession: | NP_001171737.1 |
UniProt Related Accession: | Q8TCZ2 |
Molecular Weight: | 28kDa |
NCBI Full Name: | CD99 antigen-like protein 2 isoform 4 |
NCBI Synonym Full Names: | CD99 molecule like 2 |
NCBI Official Symbol: | CD99L2 |
NCBI Official Synonym Symbols: | CD99B; MIC2L1 |
NCBI Protein Information: | CD99 antigen-like protein 2 |
UniProt Protein Name: | CD99 antigen-like protein 2 |
UniProt Synonym Protein Names: | MIC2-like protein 1; CD_antigen: CD99 |
Protein Family: | CD99 antigen-like protein |
UniProt Gene Name: | CD99L2 |
UniProt Entry Name: | C99L2_HUMAN |
Antibodies |
Anti-CD99L2 Antibody (CAB15907) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
Recommended Products |
Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |