Carboxypeptidase A2/CPA2 Recombinant Protein (RPES2616)

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SKU:
RPES2616
Product type:
Recombinant Protein
Host species:
HEK293 Cells
Reactivity:
Human
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Description

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Carboxypeptidase A2/CPA2 Recombinant Protein

Carboxypeptidase A2 ( CPA2 ) is a secreted pancreatic procarboxy -peptidase, and cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group. The hydrolytic action of CPA2 was identified with a preference towards long substrates with aromatic amino acids in their C-terminal end, particularly tryptophan. CPA2 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. Three different forms of human pancreatic procarboxypeptidase A have been isolated, and the A1 and A2 forms are always secreted as monomeric proteins with different biochemical properties.

Product Name:Carboxypeptidase A2/CPA2 Recombinant Protein (RPES2616)
Product Code:RPES2616
Size:10µg
Species:Human
Expressed Host:HEK293 Cells
Synonyms:Carboxypeptidase A2, CPA2
Accession:NP_001860.2
Sequence:Met 1-Tyr 417
Fusion tag:C-His
Activity:Measured by its ability to cleave a colorimetric peptide substrate, N-acetyl-Phe-Thiaphe-OH (N-Ac-PSP, Peptide International's Catalog# STP-3621-PI), in the presence of 5,5'Dithio-bis (2-nitrobenzoic acid) (DTNB), as measured using the wavelength at 405 nm and the extinction coefficient of 13,260 M-1 cm-The specific activity is >4,000 pmoles/min/µg.
Endotoxin:<1.0 EU per µg as determined by the LAL method.
Protein Construction:A DNA sequence encoding the pre pro form of human CPA2 (NP_001860.2) (Met 1-Tyr 417) was expressed with a C-terminal polyhistidine tag.
Purity:> 90 % as determined by reducing SDS-PAGE.
Mol Mass:46 kDa
AP Mol Mass:46 kDa
Formulation:Lyophilized from sterile 25mM Tris, 0.15mM NaCl, pH 7.4
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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