BRCC3 Antibody (PACO43688)
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주문- SKU:
- PACO43688
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | BRCC3 Antibody (PACO43688) |
Antibody SKU: | PACO43688 |
Size: | 50ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:2000, IHC:1:20-1:200 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Lys-63-specific deubiquitinase BRCC36 protein (1-316AA) |
Form: | Liquid |
Storage Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Purification Method: | Antigen Affinity Purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western blot. All lanes: BRCC3 antibody at 3µg/ml. Lane 1: 293T whole cell lysate. Lane 2: MCF-7 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 37, 34, 24, 29 kDa. Observed band size: 37 kDa. | |
Immunohistochemistry of paraffin-embedded human kidney tissue using PACO43688 at dilution of 1:100. | |
Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using PACO43688 at dilution of 1:100. |
Background: | Metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not have activity toward 'Lys-48'-linked polyubiquitin chains. Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). In the BRCA1-A complex, it specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double-strand breaks (DSBs). Catalytic subunit of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates. Mediates the specific 'Lys-63'-specific deubiquitination associated with the COP9 signalosome complex (CSN), via the interaction of the BRISC complex with the CSN complex. |
Synonyms: | Lys-63-specific deubiquitinase BRCC36 (EC 3.4.19.-) (BRCA1-A complex subunit BRCC36) (BRCA1/BRCA2-containing complex subunit 3) (BRCA1/BRCA2-containing complex subunit 36) (BRISC complex subunit BRCC36), BRCC3, BRCC36 C6.1A CXorf53 |
UniProt Protein Function: | BRCC3: Metalloprotease that specifically cleaves 'Lys-63'- linked polyubiquitin chains. Does not have activity toward 'Lys- 48'-linked polyubiquitin chains. Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). In the BRCA1-A complex, it specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double- strand breaks (DSBs). Catalytic subunit of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates. Mediates the specific 'Lys-63'- specific deubiquitination associated with the COP9 signalosome complex (CSN), via the interaction of the BRISC complex with the CSN complex. A chromosomal aberration involving BRCC3 is a cause of pro-lymphocytic T-cell leukemia (T-PLL). Translocation t(X;14)(q28;q11) with TCRA. Belongs to the peptidase M67A family. BRCC36 subfamily. 5 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Ubiquitin conjugating system; Oncoprotein; EC 3.4.19.-; Ubiquitin-specific protease Chromosomal Location of Human Ortholog: Xq28 Cellular Component: nucleoplasm; nuclear ubiquitin ligase complex; nucleus; ubiquitin ligase complex Molecular Function:protein binding; metallopeptidase activity; metal ion binding; ubiquitin-specific protease activity; polyubiquitin binding; enzyme regulator activity Biological Process: positive regulation of DNA repair; regulation of catalytic activity; double-strand break repair; response to ionizing radiation; G2/M transition DNA damage checkpoint; proteolysis; response to X-ray |
NCBI Summary: | This gene encodes a subunit of the BRCA1-BRCA2-containing complex (BRCC), which is an E3 ubiquitin ligase. This complex plays a role in the DNA damage response, where it is responsible for the stable accumulation of BRCA1 at DNA break sites. The component encoded by this gene can specifically cleave Lys 63-linked polyubiquitin chains, and it regulates the abundance of these polyubiquitin chains in chromatin. The loss of this gene results in abnormal angiogenesis and is associated with syndromic moyamoya, a cerebrovascular angiopathy. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 5. [provided by RefSeq, Jun 2011] |
UniProt Code: | P46736 |
NCBI GenInfo Identifier: | 20532383 |
NCBI Gene ID: | 79184 |
NCBI Accession: | P46736.2 |
UniProt Secondary Accession: | P46736,Q16107, Q53YX5, Q9BTZ6, A6QRF8, A6QRF9, A8MUX5 A8MWH0, A9Z1Y0, A9Z1Y5, B1B062, B4DQN7, |
UniProt Related Accession: | P46736 |
Molecular Weight: | 316 |
NCBI Full Name: | Lys-63-specific deubiquitinase BRCC36 |
NCBI Synonym Full Names: | BRCA1/BRCA2-containing complex, subunit 3 |
NCBI Official Symbol: | BRCC3 |
NCBI Official Synonym Symbols: | C6.1A; BRCC36; CXorf53 |
NCBI Protein Information: | lys-63-specific deubiquitinase BRCC36; BRISC complex subunit BRCC36; BRCA1-A complex subunit BRCC36; BRCA1/BRCA2-containing complex subunit 36 |
UniProt Protein Name: | Lys-63-specific deubiquitinase BRCC36 |
UniProt Synonym Protein Names: | BRCA1-A complex subunit BRCC36; BRCA1/BRCA2-containing complex subunit 3; BRCA1/BRCA2-containing complex subunit 36; BRISC complex subunit BRCC36 |
Protein Family: | Lys-63-specific deubiquitinase |
UniProt Gene Name: | BRCC3 |
UniProt Entry Name: | BRCC3_HUMAN |