BMI1 Antibody (PACO31664)
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주문- SKU:
- PACO31664
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
Antibody Name: | BMI1 Antibody (PACO31664) |
Antibody SKU: | PACO31664 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:20-1:200 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Polycomb complex protein BMI-1 protein (1-247AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western blot All lanes: BMI1 antibodyt 2µg/ml Lane 1: EC109 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 37 kDa Observed band size: 70 kDa . | |
Immunohistochemistry of paraffin-embedded human kidney tissue using PACO31664 at dilution of 1:100. | |
Immunohistochemistry of paraffin-embedded human glioma using PACO31664 at dilution of 1:100. |
Background: | Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. The complex composed of RNF2, UB2D3 and BMI1 binds nucleosomes, and has activity only with nucleosomal histone H2A. In the PRC1-like complex, regulates the E3 ubiquitin-protein ligase activity of RNF2/RING2. |
Synonyms: | Polycomb complex protein BMI-1 (Polycomb group RING finger protein 4) (RING finger protein 51), BMI1, PCGF4 RNF51 |
UniProt Protein Function: | BMI1: Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2. Component of a PRC1-like complex. Interacts with RING1 and RING2. Interacts vwith CBX7 and CBX8. Interacts with SPOP. Part of a complex consisting of BMI1, CUL3 and SPOP. Interacts with E4F1. |
UniProt Protein Details: | Protein type:Ubiquitin ligase; Ubiquitin conjugating system; Ligase; EC 6.3.2.-; Oncoprotein; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 10p11.23 Cellular Component: nucleoplasm; heterochromatin; nuclear body; cytoplasm; PcG protein complex; nucleus; ubiquitin ligase complex Molecular Function:protein binding; zinc ion binding; sequence-specific DNA binding; ubiquitin-protein ligase activity; chromatin binding Biological Process: transcription, DNA-dependent; in utero embryonic development; positive regulation of immature T cell proliferation in the thymus; negative regulation of transcription from RNA polymerase II promoter; humoral immune response; embryonic skeletal morphogenesis; segment specification; rostrocaudal neural tube patterning; positive regulation of fibroblast proliferation; regulation of gene expression; positive regulation of ubiquitin-protein ligase activity; histone ubiquitination; positive regulation of B cell proliferation; DNA methylation; somatic stem cell division; hemopoiesis; histone acetylation; brain development |
NCBI Summary: | This locus represents naturally occurring read-through transcription between the neighboring COMM domain-containing protein 3 and polycomb complex protein BMI-1 genes on chromosome 10. The read-through transcript produces a fusion protein that shares sequence identity with each individual gene product. [provided by RefSeq, Feb 2011] |
UniProt Code: | P35226 |
NCBI GenInfo Identifier: | 22258801 |
NCBI Gene ID: | 100532731 |
NCBI Accession: | P35226.2 |
UniProt Secondary Accession: | P35226,Q16030, Q5T8Z3, Q96F37, |
Molecular Weight: | 36,949 Da |
NCBI Full Name: | Polycomb complex protein BMI-1 |
NCBI Synonym Full Names: | COMMD3-BMI1 readthrough |
NCBI Official Symbol: | COMMD3-BMI1 |
NCBI Protein Information: | COMMD3-BMI1 read-through protein |
UniProt Protein Name: | Polycomb complex protein BMI-1 |
UniProt Synonym Protein Names: | Polycomb group RING finger protein 4; RING finger protein 51 |
Protein Family: | Polycomb complex protein |
UniProt Gene Name: | BMI1 |
UniProt Entry Name: | BMI1_HUMAN |
Antibodies |
Anti-BMI1 Antibody (CAB0211) |
Anti-BMI1 Antibody (CAB13472) |
Anti-Bmi1 Antibody (CAB17914) |
BMI1 Antibody (PACO08026) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
Recommended Products |
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Anti-HRP-conjugated Beta Actin Antibody (CABC028) |