ATRX Antibody (PACO45690)
제품 코드를 사용하여 Assay Genie 메인 사이트를 통해 주문합니다.
주문- SKU:
- PACO45690
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
Antibody Name: | ATRX Antibody (PACO45690) |
Antibody SKU: | PACO45690 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:20-1:200, IF:1:50-1:200 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Transcriptional regulator ATRX protein (2161-2443AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Immunohistochemistry of paraffin-embedded human kidney tissue using PACO45690 at dilution of 1:100. | |
Immunofluorescent analysis of Hela cells using PACO45690 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). | |
Immunohistochemistry of paraffin-embedded human pancreatic cancer using PACO45690 at dilution of 1:100. |
Background: | Involved in transcriptional regulation and chromatin remodeling. Facilitates DNA replication in multiple cellular environments and is required for efficient replication of a subset of genomic loci. Binds to DNA tandem repeat sequences in both telomeres and euchromatin and in vitro binds DNA quadruplex structures. May help stabilizing G-rich regions into regular chromatin structures by remodeling G4 DNA and incorporating H3.3-containing nucleosomes. Catalytic component of the chromatin remodeling complex ATRX:DAXX which has ATP-dependent DNA translocase activity and catalyzes the replication-independent deposition of histone H3.3 in pericentric DNA repeats outside S-phase and telomeres, and the in vitro remodeling of H3.3-containing nucleosomes. Its heterochromatin targeting is proposed to involve a combinatorial readout of histone H3 modifications (specifically methylation states of H3K9 and H3K4) and association with CBX5. Involved in maintaining telomere structural integrity in embryonic stem cells which probably implies recruitment of CBX5 to telomers. Reports on the involvement in transcriptional regulation of telomeric repeat-containing RNA (TERRA) are conflicting; according to a report, it is not sufficient to decrease chromatin condensation at telomers nor to increase expression of telomeric RNA in fibroblasts. May be involved in telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines. Acts as negative regulator of chromatin incorporation of transcriptionally repressive histone H2AFY, particularily at telomeres and the alpha-globin cluster in erythroleukemic cells. Participates in the allele-specific gene expression at the imprinted IGF2/H19 gene locus. On the maternal allele, required for the chromatin occupancy of SMC1 and CTCTF within the H19 imprinting control region (ICR) and involved in esatblishment of histone tails modifications in the ICR. May be involved in brain development and facial morphogenesis. Binds to zinc-finger coding genes with atypical chromatin signatures and regulates its H3K9me3 levels. Forms a complex with ZNF274, TRIM28 and SETDB1 to facilitate the deposition and maintenance of H3K9me3 at the 3' exons of zinc-finger genes. |
Synonyms: | Transcriptional regulator ATRX (EC 3.6.4.12) (ATP-dependent helicase ATRX) (X-linked helicase II) (X-linked nuclear protein) (XNP) (Znf-HX), ATRX, RAD54L XH2 |
UniProt Protein Function: | ATRX: a global transcriptional regulator. Belongs to the SNF2 family of proteins, many of which modify gene expression via chromatin remodeling activity. Involved in the developmental silencing of imprinted genes in the brain. Contains one PxVxL motif, which is required for interaction with chromoshadow domains. This motif requires additional residues at -7, -6, +4 and +5 relative to the central V which contact the chromoshadow domain. Constitutive mutations in ATRX are associated with brain, facial, and genital abnormalities, and alpha thalassemia. Acquired mutations in ATRX have been observed in preleukemic conditions. Six alternatively spliced human isoforms have been described. |
UniProt Protein Details: | Protein type:DNA repair, damage; EC 3.6.4.12; Helicase; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: Xq21.1 Cellular Component: centric heterochromatin; condensed nuclear chromosome, centromeric region; nuclear body; nuclear chromosome, telomeric region; nuclear heterochromatin; nuclear subtelomeric heterochromatin; nucleoplasm; nucleus; PML body Molecular Function:ATP binding; chromatin binding; chromo shadow domain binding; DNA binding; DNA helicase activity; DNA translocase activity; helicase activity; histone binding; metal ion binding; methylated histone residue binding; protein binding Biological Process: chromatin remodeling; covalent chromatin modification; DNA damage response, signal transduction by p53 class mediator; DNA duplex unwinding; DNA methylation; DNA recombination; DNA repair; DNA replication-independent nucleosome assembly; forebrain development; meiotic spindle organization; multicellular organism growth; negative regulation of maintenance of mitotic sister chromatid cohesion, telomeric; negative regulation of telomeric RNA transcription from RNA pol II promoter; nucleosome assembly; positive regulation of nuclear cell cycle DNA replication; positive regulation of telomere maintenance; positive regulation of telomeric RNA transcription from RNA pol II promoter; positive regulation of transcription from RNA polymerase II promoter; post-embryonic forelimb morphogenesis; regulation of histone H3-K9 trimethylation; regulation of transcription, DNA-templated; replication fork processing; seminiferous tubule development; Sertoli cell development; spermatogenesis; transcription, DNA-dependent Disease: Alpha-thalassemia Myelodysplasia Syndrome; Alpha-thalassemia/mental Retardation Syndrome, X-linked; Mental Retardation-hypotonic Facies Syndrome, X-linked, 1 |
NCBI Summary: | The protein encoded by this gene contains an ATPase/helicase domain, and thus it belongs to the SWI/SNF family of chromatin remodeling proteins. This protein is found to undergo cell cycle-dependent phosphorylation, which regulates its nuclear matrix and chromatin association, and suggests its involvement in the gene regulation at interphase and chromosomal segregation in mitosis. Mutations in this gene are associated with X-linked syndromes exhibiting cognitive disabilities as well as alpha-thalassemia (ATRX) syndrome. These mutations have been shown to cause diverse changes in the pattern of DNA methylation, which may provide a link between chromatin remodeling, DNA methylation, and gene expression in developmental processes. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2017] |
UniProt Code: | P46100 |
NCBI GenInfo Identifier: | 530788276 |
NCBI Gene ID: | 546 |
NCBI Accession: | NP_000480.3 |
UniProt Secondary Accession: | P46100,P51068, Q15886, Q59FB5, Q59H31, Q5H9A2, Q5JWI4 Q7Z2J1, Q9H0Z1, Q9NTS3, D3DTE2, |
UniProt Related Accession: | P46100 |
Molecular Weight: | 151,556 Da |
NCBI Full Name: | transcriptional regulator ATRX isoform 1 |
NCBI Synonym Full Names: | ATRX, chromatin remodeler |
NCBI Official Symbol: | ATRX |
NCBI Official Synonym Symbols: | JMS; XH2; XNP; MRX52; RAD54; RAD54L; ZNF-HX |
NCBI Protein Information: | transcriptional regulator ATRX |
UniProt Protein Name: | Transcriptional regulator ATRX |
UniProt Synonym Protein Names: | ATP-dependent helicase ATRX; X-linked helicase II; X-linked nuclear protein; XNP; Znf-HX |
Protein Family: | Transcriptional regulator |
UniProt Gene Name: | ATRX |