ATP6V1G2 Antibody (PACO25156)
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주문- SKU:
- PACO25156
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
Antibody Name: | ATP6V1G2 Antibody (PACO25156) |
Antibody SKU: | PACO25156 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
Species Reactivity: | Human, Mouse, Rat |
Immunogen: | Recombinant Human V-type proton ATPase subunit G 2 protein (1-118AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Western blot All lanes: ATP6V1G2 antibody at 12µg/ml Lane 1: Rat heart tissue Lane 2: Mouse spleen tissue Lane 3: Hela whole cell lysate Lane 4: HepG2 whole cell lysate Lane 5: A549 whole cell lysate Lane 6: HT29 whole cell lysate Lane 7: K562 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 14, 9 kDa Observed band size: 14 kDa . | |
Immunofluorescence staining of A549 cells with PACO25156 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). | |
Immunohistochemistry of paraffin-embedded human brain tissue using PACO25156 at dilution of 1:100. |
Background: | Catalytic subunit of the peripheral V1 complex of vacuolar ATPase (V-ATPase). V-ATPase is responsible for acid, fying a variety of intracellular compartments in eukaryotic cells. |
Synonyms: | V-type proton ATPase subunit G 2 (V-ATPase subunit G 2) (V-ATPase 13 kDa subunit 2) (Vacuolar proton pump subunit G 2), ATP6V1G2, ATP6G ATP6G2 NG38 |
UniProt Protein Function: | |
UniProt Protein Details: | |
NCBI Summary: | This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular compartments of eukaryotic cells. V-ATPase dependent acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of three V1 domain G subunit proteins. This gene had previous gene symbols of ATP6G and ATP6G2. Alternatively spliced transcript variants encoding different isoforms have been described. Read-through transcription also exists between this gene and the downstream DEAD (Asp-Glu-Ala-Asp) box polypeptide 39B (DDX39B) gene. [provided by RefSeq, Feb 2011] |
UniProt Code: | O95670 |
NCBI GenInfo Identifier: | 7388349 |
NCBI Gene ID: | 534 |
NCBI Accession: | |
UniProt Secondary Accession: | O95670 |
UniProt Related Accession: | O95670 |
Molecular Weight: | |
NCBI Full Name: | V-type proton ATPase subunit G 2 |
NCBI Synonym Full Names: | ATPase H+ transporting V1 subunit G2 |
NCBI Official Symbol: | ATP6V1G2 |
NCBI Official Synonym Symbols: | NG38; ATP6G; VMA10; ATP6G2 |
NCBI Protein Information: | V-type proton ATPase subunit G 2 |
UniProt Protein Name: | V-type proton ATPase subunit G 2 |
UniProt Synonym Protein Names: | Vacuolar proton pump subunit G 2; V-ATPase 13 kDa subunit 2 |
Protein Family: | V-type proton ATPase |
UniProt Gene Name: | ATP6V1G2 |
UniProt Entry Name: | VATG2_HUMAN |